生物合成纳米硒治疗非酒精性脂肪肝病的机制研究  

Mechanism of Biosynthesized Selenium-Nanoparticles in Treating Non-Alcoholic Fatty Liver Disease

作　　者：张展鹏 谢宁宁 夏伟年 杜豪杰 牟覃恩 樊斌 ZHANG Zhanpeng;XIE Ningning;XIA Weinian;DU Haojie;MOU Qinen;FAN Bin(Hubei Minzu Universitysity,Enshi 445000,China;Hospital of Enshi Autonomous Prefecture,Enshi 445000,China;Hubei Selenium and Human Health Institute,Enshi 445000,China)

机构地区：[1]湖北民族大学,湖北恩施445000 [2]恩施自治州中心医院,湖北恩施445000 [3]湖北硒与人体健康研究院,湖北恩施445000

出　　处：《湖北民族大学学报（医学版）》2024年第4期8-13,共6页Journal of Hubei Minzu University(Medical Edition)

基　　金：恩施州科技计划项目(D20220074);湖北民族大学科研创新项目(MYK2024044)。

摘　　要：目的研究生物合成纳米硒对小鼠非酒精性脂肪肝病(non-alcoholic fatty liver disease,NAFLD)的作用与机制。方法C57BL/6J雄性小鼠30只,使用高脂、低蛋氨酸、胆碱缺乏(CDAHFD)饲料诱导小鼠NAFLD。小鼠随机分为3组:空白组(正常饲养6周)、模型组(CDAHFD饲料喂养6周),治疗组(CDAHFD饲料喂养6周+7.5μg/20 g纳米硒灌胃6周,每周1次)。记录小鼠体质量变化情况,实验周期结束后禁食12 h,解剖取材,检测血清肝功能、血脂指标;肝脏HE、油红O、马松染色观察肝脏病理学形态、脂质沉积及肝纤维化程度。qRT-PCR检测脂肪代谢、炎性细胞因子的基因表达水平。结果与空白组相比,模型组小鼠谷丙转氨酶(ALT)、谷草转氨酶(AST)、甘油三酯(TG)、总胆红素(TB)显著增高(P<0.01),肝脏质量、肝脏系数显著升高(P<0.01),肝脏病理切片显示大量细胞肿胀变形、红色脂质沉积、胶原纤维生成,脂质代谢与炎症因子mRNA表达升高(P<0.05),与模型组比较,治疗组小鼠ALT、AST、TG、TB明显下降(P<0.05),肝脏质量、肝脏系数降低(P<0.05),肝脏病理切片提示细胞肿胀变形减少、脂质沉积降低、纤维化程度下降,脂质代谢与炎症因子mRNA表达下降(P<0.05)。结论生物合成纳米硒可有效改善高脂饮食诱导的小鼠NAFLD,减少脂质沉积、肝脏炎症与纤维化。Objective To investigate the effects and mechanisms of Bio-synthesized Selenium-nanoparticles on non-alcoholic fatty liver disease(NAFLD)in mice.Methods Thirty male C57BL/6J mice were used to induce NAFLD in mice using a high-fat,low-methionine,and choline-deficient(CDAHFD)diet.The mice were randomly divided into three groups:the blank group(Con,normal feeding for 6 weeks),the model group(CDAHFD,CDAHFD diet feeding for 6 weeks and PBS gavage),and the treatment group(CDAHFD+SeNPs,CDAHFD diet feeding for 6 weeks and 7.5μg/20 g nano-selenium gavage once a week for 6 weeks).The weight changes of the mice were recorded.After the end of the experimental period,the mice were fasted for 12 hours,dissected for sample collection,and the serum liver function and lipid indicators were detected.The liver was stained with HE,oil red O,and Masson staining for observing the pathological morphology,lipid deposition,and the degree of liver fibrosis.qRT-PCR was utilized to detect the levels of fat metabolism and inflammatory cytokines.Results Compared with the Con group,the liver function indicators of ALT and AST in the model group were significantly increased(P<0.01),as were the blood lipid indicators of TG and TB(P<0.01).The liver weight and liver coefficient were also significantly increased(P<0.01).Histological sections of the liver showed a large number of cells swollen and deformed,red lipid deposits,and collagen fibers.The mRNA expression of lipid metabolism and inflammatory factors was increased(P<0.05).Compared with the model group,the liver function indicators of ALT and AST,as well as the blood lipid indicators of TG and TB,were significantly decreased(P<0.01)in the nano-selenium treatment group.The liver weight and liver coefficient were also significantly lower(P<0.01).Histological sections of the liver showed a reduction in cell swelling and deformation,a decrease in lipid deposits,and a lower degree of fibrosis.The mRNA expression of lipid metabolism and inflammatory factors was also decreased(P<0.05).Conclusions B

关 键 词：纳米硒 非酒精性脂肪肝病 肝脏炎症 肝纤维化 

分 类 号：R575.5[医药卫生—消化系统]