2株益生菌体外特性及对糖尿病大鼠血糖影响  

In vitro properties of two probiotics and their effects on blood glucose in diabetic rats

作　　者：张迪 王婷婷 张晶晶 葛阳阳 李守远 徐英 张世奇 尚楠 ZHANG Di;WANG Tingting;ZHANG Jingjing;GE Yangyang;LI Shouyuan;XU Ying;ZHANG Shiqi;SHANG Nan(Coree Beijing Co.Ltd.,Beijing 101318,China;Beijing Hanmi pharmaceutical Co.Ltd.,Beijing 101312,China;College of Engineering,China Agricultural University,Beijing 100083,China)

机构地区：[1]北京科丽科技有限公司,北京101318 [2]北京韩美药品有限公司,北京101312 [3]中国农业大学工学院,北京100083

出　　处：《中国乳品工业》2024年第11期31-37,共7页China Dairy Industry

基　　金：国家自然科学基金项目(32201994)。

摘　　要：文章研究植物乳植杆菌HOM3201和动物双歧杆菌乳亚种HOM1119的益生特性及对糖尿病大鼠血糖等指标的影响,筛选具有降血糖作用的益生菌。通过体外试验,测定2株益生菌对模拟胃肠液耐受性、产酸能力、抑制致病菌能力、抗氧化活性、对二肽基肽酶一4和α-葡萄糖苷酶抑制性及促NCI-H716细胞分泌胰高血糖素样肽-1的活性;并通过胰岛素抵抗糖/脂代谢紊乱大鼠模型试验,分析菌株对大鼠糖尿病的改善作用。结果表明,HOM3201和HOM1119菌株耐受模拟胃肠液(存活率>95%),可产生高含量乳酸和乙酸,对5种食源性致病菌生长均具有抑制作用,并且具有清除自由基能力;对二肽基肽酶-4和α-葡萄糖苷酶的抑制率及促胰高血糖素样肽-1的分泌量均显著高于对照菌株GG(P<0.05)。灌服益生菌粉41d后,模型组、HOM3201组和HOM1119组大鼠空腹血糖值分别为8.3、7.9和8.0,表明HOM3201和HOM1119菌株均可显著降低糖尿病模型大鼠空腹血糖水平(P<0.05)。To screen probiotics with hypoglycemic effect,the probiotic properties of Lactiplantibacillus plantarum HOM3201 and Bifidobacterium animalis subsp.lactis HOM1119 and their effects on indexes such as blood glucose level in diabetic rats were studied in this paper.The tolerance of simulated gastrointestinal fluid,the capacity of acid production,the ability of inhibiting pathogenic bacteria,the antioxidant activity,the inhibitory activity of dipeptidyl peptidase-4 andα-glucosidase and the capacity of promoting the secretion of glucagon-like peptide-1 by NCI-H716 cells were determined in vitro.The effects on alleviating diabetes in rats were evaluated by using an insulin resistance glucose/lipid metabolism disorder rat model.The results showed that HOM3201 and HOM1119 strains:tolerated simulated gastric and intestinal juices(survival rate>95%),produced high contents of lactic acid and acetic acid,significantly inhibited the growths of five foodborne pathogens,scavenged free radicals,the inhibition rates of dipeptidyl peptidase-4 andα-glucosidase and the capacity of promoting the secretion level of glucagon-like peptide-1 of NCI-H716 cells were significantly higher than those of the control strain GG(P<0.05).After 41 days of oral administration,the fasting blood glucose values of model group,HOM3201 and HOM1119 were 8.3,7.9 and 8.0,respectively,indicating that both HOM3201 and HOM1119 strains could significantly reduce the fasting blood glucose levels in diabetic model rats(P<0.05).

关 键 词：植物乳植杆菌 动物双歧杆菌乳亚种 益生特性 二肽基肽酶-4 胰高血糖素样肽-1 降血糖 

分 类 号：TS201.3[轻工技术与工程—食品科学]