可可毛色二孢菌侵染芒果果实时茉莉酸和水杨酸信号通路的抗性响应  

Resistance Response of the Jasmonic Acid and Salicylic Acid Signaling Pathways in Mango Fruits Infected with Lasodiplodia theobromae

作　　者：屈湘乡 胡美姣[1] 孙进华[1] 弓德强[1] 高兆银[1] 陈更新 李敏[1] QU Xiangxiang;HU Meijiao;SUN Jinhua;GONG Deqiang;GAO Zhaoyin;CHEN Gengxin;LI Min(Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China;School of Life Sciences,Hainan University,Haikou 570228,China;School of Tropical Agriculture and Forestry,Hainan University,Haikou 570228,China)

机构地区：[1]中国热带农业科学院环境与植物保护研究所,海南海口571101 [2]海南大学生命科学学院,海南海口570228 [3]海南大学热带农林学院,海南海口570228

出　　处：《食品科学》2025年第1期11-17,共7页Food Science

基　　金：海南省自然科学基金高层次人才项目(321RC620,322RC753,320RC688);海南省重点研发计划项目(ZDYF2021XDNY287)。

摘　　要：为明确可可毛色二孢菌(Lasiodiplodia theobromae)侵染芒果果实过程中茉莉酸(jasmonic acid,JA)和水杨酸(salicylic acid,SA)信号通路的响应模式,分析L.theobromae与芒果果实互作过程中侵染菌丝体JA和SA的合成、芒果果实中JA和SA的合成及其信号通路相关基因和抗性相关基因表达情况。结果表明:L.theobromae在侵染芒果果实时JA和SA产量逐渐增加;接种的芒果果实中JA产量显著升高,SA产量则显著下降(P<0.05);JA信号通路相关基因茉莉酸异亮氨酸合成酶1(MiJAR1)、丙二烯氧化物合成酶(MiAOS)和髓细胞增生蛋白2(MiMYC2)基因在早期表达快速上调,后期表达下调,冠菌素敏感蛋白1(MiCOI1)基因表达持续下调;SA信号通路相关基因异分支酸合酶(MiICS)基因表达下调,病程相关基因非表达子(MiNPR1)基因早期表达上调,后期表达下调;苯丙氨酸解氨酶(MiPAL)、过氧化物酶(MiPOD)和β-1,3-葡聚糖酶(MiGLU)基因前期表达上调,几丁质酶(MiCHI)基因表达12 h后持续上调。上述结果表明,在侵染芒果果实过程中,L.theobromae激活了果实JA的合成及其信号转导,但持续抑制了果实SA的合成及其信号转导。In order to explore the response patterns of the jasmonic acid(JA)and salicylic acid(SA)signaling pathways in mango fruits to Lasiodiplodia theobromae,we analyzed the synthesis of JA and SA in L.theobromae mycelia infecting the fruit and in the infected fruit as well as the expression of genes involved in the JA and SA signaling pathways and the expression of resistance-related genes.The results showed that the production of JA and SA in the mycelia gradually increased with increasing infection time.JA production increased significantly in the inoculated fruit,while SA production decreased significantly with increasing storage time(P<0.05).The expression of genes related to JA signaling pathway,such as jasmonate resistant 1(MiJAR1),allene oxide synthase(MiAOS),and myelocytomatosis 2(MiMYC2)were rapidly up-regulated at the early stage and down-regulated at the late stage.The gene expression of coronatin insensitive 1(MiCOI1)was continuously down-regulated.The gene expression of isochorismate synthase(MiICS),a SA signaling pathway-related gene,was down-regulated,while the gene expression of non-expressor of pathogenesis-related genes 1(MiNPR1)was up-regulated at the early stage and down-regulated at the late stage.The expression of resistance-related genes,such as phenylalanine ammonia lyase(MiPAL),peroxidase(MiPOD),andβ-1,3-glucanase(MiGLU)were up-regulated at the early stage,while the expression of chitinase gene(MiCHI)was continuously up-regulated after 12 h.Overall,these results indicate that infection with L.theobromae activates the synthesis of JA and its signal transduction,but inhibits the synthesis and signal transduction of SA in mango fruits.

关 键 词：茉莉酸 水杨酸 芒果 可可毛色二孢 采后病害 

分 类 号：S436.6[农业科学—农业昆虫与害虫防治]