基于关联分析挖掘小麦SDS沉降值相关候选基因及KASP标记开发  

Discovering of Candidate Genes for Wheat SDS-Sedimentation Value Using Association Study and Development of KASP Marker

作　　者：刘海霞 张寅辉 庄蕾 郭梦娇 赵李 吴美娟 侯健[1] 李甜[1] 刘红霞[1] 张学勇[1] 郝晨阳[1] LIU Haixia;ZHANG Yinhui;ZHUANG Lei;GUO Mengjiao;ZHAO Li;WU Meijuan;HOU Jian;LI Tian;LIU Hongxia;ZHANG Xueyong;HAO Chenyang(State Key Laboratory of Crop Gene Resources and Breeding,National Key Facility for Crop Gene Resources and Genetic Improvement,Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]中国农业科学院作物科学研究所,作物基因资源与育种全国重点实验室,农作物基因资源与基因改良国家重大科学工程,北京100081

出　　处：《中国农业科技导报》2024年第12期18-29,共12页Journal of Agricultural Science and Technology

基　　金：农业生物育种国家科技重大专项(2022ZD04017);国家重点研发计划项目(2023YFD1200404)。

摘　　要：SDS沉降值是小麦品质育种的一项重要指标。对145份小麦品种的SDS沉降值进行了全基因组关联分析,在染色体3DL末端鉴定到成簇的显著信号,结合peak SNP的位置与LD衰减距离锁定587.514~589.514 Mb为候选区段;利用重测序数据(http://wheat.cau.edu.cn/WheatUnion/)发掘该区段内候选基因的变异位点,再对SDS沉降值的候选基因进行关联分析。综合关联结果、基因注释和基因表达分析,获得沉降值相关候选基因TraesCS3D03G1092400,命名为TaAGAP-3D。基于该基因的多态性SNP位点开发了可供育种利用的KASP标记Sv-3D-KASP,发现TaAGAP-3D(C)为SDS沉降值相关的优异等位变异。研究结果将为小麦SDS沉降值的遗传改良提供有效分子标记,同时也为进一步克隆品质基因提供参考。SDS sedimentation value is an important indicator for wheat quality breeding.In this study,genome-wide association study(GWAS)on SDS-sedimentation value was performed in 145 wheat accessions.The results revealed that a cluster of significant signals was identified at the end of 3DL,and combined with the position of the peak SNP and the distance of LD decay,the candidate interval 587.514~589.514 Mb was further determined.Then,variation sites of candidate genes within this region were explored based on the resequencing data(http://wheat.cau.edu.cn/WheatUnion/),and candidate gene association study(CGAS)was conducted in combination with SDSsedimentation value.By integrating association results,gene annotation and gene expression analysis,TraesCS3D03G1092400 was identified as the SDS-sedimentation value-related candidate gene,and was tentatively named TaAGAP-3D.Additionally,a KASP marker Sv-3D-KASP for breeding was developed based on the polymorphic SNP of this gene,and found that TaAGAP-3D(C)was a favorable allele for SDS-sedimentation value.Altogether,these findings provide an effective molecular marker for the genetic improvement of SDS-sedimentation value in wheat,and also provide a reference for further cloning of quality genes.

关 键 词：小麦 SDS沉降值 全基因组关联分析 候选基因关联分析 KASP标记 

分 类 号：S511[农业科学—作物学]