基于全基因组测序的菌株发酵粘液乳杆菌(Limosilactobacillus fermentum)FUA033的安全性和益生特性分析  

Safety and probiotic characteristics of Limosilactobacillus fermentum FUA033 based on complete genome analysis

作　　者：母淑婷 华梓延 赵双双 房耀维 杨光 侯晓月 宫艳艳 桂家进 刘姝 MU Shuting;HUA Ziyan;ZHAO Shuangshuang;FANG Yaowei;YANG Guang;HOU Xiaoyue;GONG Yanyan;GUI Jiajin;LIU Shu(Co-Innovation Center of Jiangsu Marine Bio-industry Technology,Lianyungang 222005,China;School of Marine Food and Bioengineering Jiangsu Ocean University,Lianyungang 222005,China;Lianyungang Food and Drug Inspection and Testing Center,Lianyungang 222005,China)

机构地区：[1]江苏省海洋生物产业技术协同创新中心,江苏连云港222005 [2]江苏海洋大学海洋食品与生物工程学院,江苏连云港222005 [3]连云港市食品药品检验检测中心,江苏连云港222005

出　　处：《食品与发酵工业》2024年第24期169-175,共7页Food and Fermentation Industries

基　　金：江苏省第五期“333工程”科研项目(BRA2019243);江苏省高等学校自然科学研究重大项目(20KJA550001);江苏省研究生科研与实践创新计划项目(KYCX2023_32);大学生创新创业训练计划立项项目(SY202311641638001)。

摘　　要：发酵粘液乳杆菌(Limosilactobacillus fermentum)FUA033可以体外转化鞣花酸生成尿石素A,具有开发为新一代益生菌的潜力。为全面了解L.fermentum FUA033的遗传信息,该研究通过PacBio和Illumina相结合对该菌株进行全基因组测序,并从基因水平对菌株潜在的安全性和益生特性进行分析。结果表明,L.fermentum FUA033基因组全长2150545 bp,GC含量51.34%,不含质粒,编码基因2215个;VFDB数据库与ARDB数据库注释结果显示L.fermentum FUA033中含有81个毒力基因和9个耐药基因,因菌株中不具有质粒和转座子,初步断定毒力因子与耐药基因不会转移;L.fermentum FUA033基因组中有耐酸基因(arcA、argH、napA等)19个,耐胆盐基因(ldhA、ykpA、pstB1等)16个,抗氧化基因(msrA、Trxs、ahpC等)11个,细胞黏附性基因(scpA、scpB、luxS)3个。研究结果初步揭示了L.fermentum FUA033潜在的安全性和益生特性,为菌株进一步的开发利用奠定了理论基础。Limosilactobacillus fermentum FUA033,a strain capable of converting ellagic acid to urolithin A in vitro,has the potential to be developed as a next-generation probiotic.In the present study,the genome of the strain was sequenced with the PacBio Sequel and Illumina platform to have a comprehensive understanding of the genetic architecture of L.fermentum FUA033.The whole genome sequence was analyzed to evaluate the safety and probiotic potential of the strain.The chromosome size of this strain was 2150545 bp with a GC content of 51.34%and 2215 protein-coding genes.No plasmid was identified.VFDB database and ARDB database annotation results showed that L.fermentum FUA033 contained 81 virulence genes and 9 resistance genes.Due to the absence of plasmids and transposons,it was tentatively concluded that the virulence factors and resistance genes would not transfer.19 acid tolerance genes(arcA,argH,napA,etc.),16 bile salt tolerance genes(ldhA、ykpA、pstB 1,etc.),11 antioxidant genes(msrA,Trxs,ahpC,etc.),and 3 cell adhesion genes were mined from the genome sequence of L.fermentum FUA033.The results preliminarily revealed the safety and probiotic properties of L.fermentum FUA033 and laid a theoretical foundation for the development and further application of the strain.

关 键 词：发酵粘液乳杆菌 尿石素A 基因组测序 安全性 益生特性 

分 类 号：TS201.3[轻工技术与工程—食品科学]