阿拉伯半乳聚糖处理对青脆李果实褐腐菌的抑制效果及其诱导抗病性机理  

Inhibitory effect of arabinogalactan treatment on postharvest brown rot of Qingcui plum and its mechanism of inducing disease resistance

作　　者：袁田 刘莹 刘玲[1,2] 韩赛影[1,2] 张婷婷 郑安祺 明建[1] 邓丽莉 YUAN Tian;LIU Ying;LIU Ling;HAN Saiying;ZHANG Tingting;ZHENG Anqi;MING Jian;DENG Lili(College of Food Science,Southwest University,Chongqing 400715,China;National Demonstration Center for Experimental Food Science and Technology Education(Southwest University),Chongqing 400715,China)

机构地区：[1]西南大学食品科学学院,重庆400715 [2]食品科学与工程国家级实验教学示范中心(西南大学),重庆400715

出　　处：《食品与发酵工业》2024年第24期254-260,共7页Food and Fermentation Industries

基　　金：农产综函【2022】35号;西南大学大学生创新训练项目(X202310635435)。

摘　　要：通过诱导果实抗病性控制果实采后病害是减少果实采后损失的重要手段。为研究阿拉伯半乳聚糖(arabinogalactan,AG)处理对采后青脆李果实褐腐病的抑制效果及其与果实抗病性的关系,该文研究了AG对褐腐病原菌(Monilinia fructicola)的直接抑制作用,以及异孔损伤接种及AG浸泡处理对青脆李果实褐腐病的控制效果,并通过测定青脆李果实酚酸含量、抗病相关酶活力及其相关基因表达量,初步阐明其提高青脆李果实抗病性的可能原因。结果表明,AG对M.fructicola的生长无抑制作用;AG处理后青脆李果实的发病率、病斑直径及自然发病率均显著低于对照(P<0.05);此外,AG处理显著提高青脆李果实酚酸含量,过氧化物酶、多酚氧化酶、β-1,3-葡聚糖酶、几丁质酶活力及多酚氧化酶、几丁质酶、病程相关蛋白相关基因表达量。综上所述,AG处理可通过提高抗病相关基因表达量,调控抗病相关酶活力及酚酸的积累,进而提高青脆李果实对褐腐病的抗病性。Controlling postharvest fruit diseases by inducing fruit disease resistance is an important means to reduce postharvest fruit losses.To investigate the inhibitory effect of arabinogalactan(AG)treatment on postharvest brown rot of Qingcui plum and its relationship with fruit disease resistance,the direct inhibitory effect of AG on Monilinia fructicola,the control effect of wound inoculation and AG soaking treatment on brown rot of plum fruits,the contents of phenolic acid,activities of enzymes,and expression level of genes related to disease resistance were determined after AG treatment.Results showed that AG did not inhibit the growth of M.fructicola,and the incidence,spot diameter,and natural incidence of M.fructicola were significantly lower than those of the control(P<0.05).In addition,AG treatment significantly increased the content of phenolic acid and the activity of peroxidase,polyphenol oxidase,β-1,3-glucanase,and chitinase,and the expression levels of genes related to peroxidase,chitinase,and disease-related proteins in plum fruit.In conclusion,AG treatment regulates the activity of disease-related enzymes and the accumulation of phenolic acids by increasing the expression level of disease-related genes,thereby improving the disease resistance of Qingcui plum and inhibiting the occurrence of postharvest diseases.

关 键 词：青脆李 阿拉伯半乳聚糖 褐腐病 诱导抗病性 采后病害 

分 类 号：TS255.3[轻工技术与工程—农产品加工及贮藏工程]