GPX4基因多态性与幽门螺杆菌感染的关联研究  

Association analysis of GPX4 gene polymorphisms with Helicobacter pylori infection

作　　者：陈雅茹 高芳[2] 董文杰[1] 褚宁 贾彦彬[1] CHEN Yaru;GAO Fang;DONG Wenjie;CHU Ning;JIA Yanbin(School of Basic Medicine and Forensic Medicine,Baotou Medical College,Baotou 014040,China;School of Medical Technologyand Anaesthesia;Clinical laboratory of the second affiliated hospital,Baotou 014040,China)

机构地区：[1]包头医学院基础医学与法医学院,内蒙古包头014040 [2]包头医学院医学技术与麻醉学院,内蒙古包头014040 [3]包头医学院第二附属医院检验科,内蒙古包头014040

出　　处：《海南医学院学报》2024年第23期1792-1797,共6页Journal of Hainan Medical University

基　　金：内蒙古自然科学基金资助项目(2024MS08079、2021MS08054);包头医学院创新团队(bycxtd-06);包头医学院科学研究基金项目(BYJJ-GDJY202313)。

摘　　要：目的:分析谷胱甘肽过氧化酶4(GPX4)基因单核苷酸多态性(SNP)与幽门螺杆菌(H.pylori)感染的相关性。方法:选取506例研究样本,采用酶联免疫吸附试验与14C尿素呼气试验检测H.pylori的感染情况,其中任意一种检测结果为阳性则判定为阳性;采用聚合酶链式反应-限制性片段长度多态性法检测GPX4基因rs4807542、rs713041、rs2074451、rs3746162的基因型分布;采用非条件性Logistic回归分析各SNP在H.pylori感染中的作用;采用SHEsis软件构建单体型,并分析其与H.pylori感染的关联性;采用广义多因子降维方法分析SNP-SNP的交互作用与H.pylori感染的关系。结果:GPX4 rs4807542、rs713041、rs2074451、rs3746162在共显性、显性、隐性和超显性模型下均与H.pylori感染无关联(P>0.05);由rs4807542、rs713041、rs2074451、rs3746162构建的单体型未发现与H.pylori感染有相关性,这四个SNP在H.pylori感染中也不存在交互作用(P>0.05)。结论:GPX4 rs4807542、rs713041、rs2074451、rs3746162可能在包头汉族人群H.pylori感染的过程中不起主要作用。Objective:To analyze the association of single nucleotide polymorphisms(SNP)in glutathione peroxidase 4(GPX4)gene with Helicobacter pylori(H.pylori)infection.Methods:A total of 506 samples were selected to detect H.pylori in-fection by enzyme-linked immunosorbent assay(ELISA)and 14C Urea breath test(UBT),a positive result of either test was deter-mined as positive;GPX4 rs4807542,rs713041,rs713041 and rs3746162 were genotyped by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)method;Unconditional logistic regression was used to analyze the association of each SNP with H.pylori infection.SHEsis software was used to constructed Haplotype to analyze its association with H.pylori in-fection.The relationship between SNP-SNP interaction and H.pylori infection was analyzed by generalized multi-factor dimen-sionality reduction method.Results:GPX4 rs4807542,rs713041,rs2074451 and rs3746162 were not associated with H.pylori in-fection in codominant,dominant,recessive,and overdominant models(P>0.05);Haplotypes constructed by rs4807542,rs713041,rs2074451 and rs3746162 were not founded to be associated with H.pylori infection,and these SNPs also did not have interaction in H.pylori infection(P>0.05).Conclusion:GPX4 rs4807542,rs713041,rs2074451 and rs3746162 may not play a major role in the process of H.pylori infection in Baotou Han population.

关 键 词：谷胱甘肽过氧化酶4 单核苷酸多态性 幽门螺杆菌 单体型 

分 类 号：R573[医药卫生—消化系统]