化州柚果瓤中柚皮苷的提取及其抗氧化活性研究  

Extraction of naringin from the pulp of Citrus grandis‘Tomentosa’and its antioxidant activity

作　　者：梁志[1,2] 刘影 甘钊生[1] LIANG Zhi;LIU Ying;GAN Zhaosheng(Department of Chemical Engineering,Maoming Vocational and Technical College,Maoming 525000;Maoming Branch,Guangdong Laboratory for Lingnan Modern Agriculture,Maoming 525028)

机构地区：[1]茂名职业技术学院化学工程系,茂名525000 [2]岭南现代农业科学与技术广东省实验室茂名分中心,茂名525028

出　　处：《中国食品添加剂》2024年第12期114-119,共6页China Food Additives

基　　金：岭南现代农业科学与技术广东省实验室茂名分中心基金资助课题(2022ZD006);2024年度茂名市科技计划立项项目(茂科字[2024]25号);广东省普通高校特色创新项目(2022KTSCX351);2023年度校级教研科研立项项目(茂职院[2023]85号:3)。

摘　　要：采用表面活性剂协同微波提取化州柚果瓤柚皮苷,在单因素试验的基础上采用正交试验优化化州柚果瓤柚皮苷的提取工艺并评价其抗氧化活性。结果表明,化州柚果瓤柚皮苷的最佳提取工艺为乙醇浓度50%、微波时间5 min、SDS质量分数1.5%、固液比1∶20(g/mL),在此条件下,化州柚果瓤柚皮苷提取得率为11.60%,RSD为1.6%;化州柚果瓤柚皮苷对DPPH具有较强的清除能力,当化州柚果瓤柚皮苷浓度≥0.40 mg/mL时,其对DPPH自由基的清除能力与Vc相当。The naringin from the pulp of Citrus grandis‘Tomentosa’was extracted using surfactant-microwave extraction method,on the basic of single-factor experiment the extraction technology of naringin from the pulp of Citrus grandis‘Tomentosa’were optimized using orthogonal test and its antioxidant activity was evaluated.The results showed that the optimal extraction process parameters for naringin from the pulp of Citrus grandis‘Tomentosa’were as follows:ethanol concentration of 50%,microwave time of 5 minutes,SDS mass fraction of 1.5%,and a solid-liquid ratio of 1∶20(g/mL).Under these conditions,the extraction rate of naringin from the pulp of Citrus grandis‘Tomentosa’was 10.60%,with an RSD of 1.6%.The naringin from the pulp of Citrus grandis‘Tomentosa’showed a strong ability to scavenge DPPH,when the concentration of naringin extracted from the pulp of Citrus grandis‘Tomentosa’was≥0.40mg/mL,its ability to scavenging DPPH free radicals was comparable to that of Vc.

关 键 词：化州柚果瓤 柚皮苷 表面活性剂 微波 正交试验 抗氧化活性 

分 类 号：TS201[轻工技术与工程—食品科学]