紫外-可见分光光度法测定饮料中柠檬黄的研究  

Study on the Determination of Lemon Yellow in Beverages by UV Visible Spectrophotometry

作　　者：王开辉 WANG Kaihui(Shenzhen Academy of Metrology&Quality Inspection,Shenzhen 518000,China)

机构地区：[1]深圳市计量质量检测研究院,广东深圳518000

出　　处：《食品安全导刊》2024年第33期51-53,共3页China Food Safety Magazine

摘　　要：采用银纳米颗粒与柠檬黄结合反应,建立了紫外-可见分光光度法测定饮料中柠檬黄的方法。通过单因素实验法筛选出最佳的测定条件为加入3.0mL银纳米颗粒分散液做反应试剂,调节体系pH值为6.0,在4℃下反应20 min后于波长400 nm处测定吸光度。经过进一步方法学验证表明,该方法线性范围较宽、线性关系、准确度与精密度良好,可用于饮料中柠檬黄含量的检测。The method for determining lemon yellow in beverages using UV visible spectrophotometry was established by combining silver nanoparticles with lemon yellow.The optimal measurement conditions were selected through a single factor experiment method,which involved adding 3.0 mL of silver nanoparticle dispersion as the reaction reagent,adjusting the pH of the system to 6.0,reacting at 4℃for 20 minutes,and measuring the absorbance at a wavelength of 400 nm.Further methodological validation has shown that this method has a wide linear range,good linear relationship,and good accuracy and precision,and can be used for the detection of lemon yellow content in beverages.

关 键 词：分光光度法 饮料 柠檬黄 

分 类 号：O657.32[理学—分析化学] TS27[理学—化学]