贻贝仿生修饰多孔磁性材料的制备及其在固定化脂肪酶中的应用  

Preparation of mussel-inspired porous magnetic materials for application in immobilized lipase

作　　者：李佥[1] 王添誉 孙西同 陈晓艺[1] 李苗 韩雨擎 曾祥冰 孙芳鸿 李宪臻[1] LI Qian;WANG Tianyu;SUN Xitong;CHEN Xiaoyi;LI Miao;HAN Yuqing;ZENG Xiangbing;SUN Fanghong;LI Xianzhen(School of Biological Engineering,Dalian Polytechnic University,Dalian 116034,China;School of Light Industry and Chemical Engineering,Dalian Polytechnic University,Dalian 116034,China)

机构地区：[1]大连工业大学生物工程学院,大连116034 [2]大连工业大学轻工与化学工程学院,大连116034

出　　处：《复合材料学报》2024年第11期6156-6169,共14页Acta Materiae Compositae Sinica

基　　金：国家自然科学基金青年基金项目(31601411,21804129,22108024);辽宁省教育厅基本科研项目(JYTMS20230423)。

摘　　要：本文制备了一种以磁性壳聚糖为基材的多孔复合材料,通过在其表面涂覆聚多巴胺涂层替代传统的交联剂,用于脂肪酶的固定化研究。该材料具有优异的孔结构和较大的比表面积,孔容积可达0.6028 mL/g,比表面积可达106.8239 m^(2)/g,经优化后固定化脂肪酶的酶活可达(7392.91±121.22)U/g-载体。进一步探究了固定化酶的酶学性质,得到最佳反应温度为50℃,最佳反应pH为7.0,制备的固定化酶具有优异的热稳定性和pH稳定性,经过5次循环使用后,该固定化酶可以保持80%以上的初始酶活,经过10次循环使用后仍能保持52%的初始酶活。最后,将固定化酶应用于生物柴油转化并优化了相关工艺参数。In this study,a porous composite material based on magnetic chitosan was prepared for lipase immobilization by applying a polydopamine coating on its surface instead of the traditional cross-linking agent.The material had excellent pore structure and large specific surface area,with a pore volume of 0.6028 mL/g and a specific surface area of 106.8239 m^(2)/g,and the highest enzyme activity of the immobilized lipase could reach up to(7392.91±121.22)U/g-carrier.In addition,the enzymatic properties of the immobilized enzyme were investigated,and the optimal reaction temperature and pH were 50℃and 7.0,respectively.Furthermore,the prepared immobilized enzyme exhibited good thermal and pH stabilities,and could maintain above 80%of the initial enzyme activity by the immobilized enzyme after five cycles of reuse,while 52%of the initial enzyme activity could be still maintained after ten cycles of reuse.Finally,the immobilized enzyme was applied in biodiesel conversion and the process was optimized.

关 键 词：磁性材料 贻贝仿生 脂肪酶 酶学性质 生物柴油转化 

分 类 号：Q814[生物学—生物工程] TB332[一般工业技术—材料科学与工程]