DNA aptamers selection and characterization for development of impedimetric aptasensor for Bacillus cereus at different growing stages  

作　　者：Nuo Duan Mingyue Ye Minghui Lu Xiaowan Chen Shijia Wu 

机构地区：[1]State Key Laboratory of Food Science and Resources,Jiangnan University,Wuxi,214122,China [2]School of Food Science and Technology,Jiangnan University,Wuxi,214122,China [3]International Joint Laboratory on Food Safety,Jiangnan University,Wuxi,214122,China

出　　处：《Advanced Agrochem》2023年第3期284-290,共7页现代农业化学（英文）

基　　金：supported by National Natural Science Fund of China(NSFC 32272449);Jiangsu Agriculture Science and Technology Innovation Fund(CX(22)3006);Guangzhou Science and Technology Project(202206010096);the Fundamental Research Funds for the Central Universities(JUSRP622025);Collaborative innovation center of food safety and quality control in Jiangsu Province.

摘　　要：The physiology(membrane integrity,intracellular esterase and redox activity)of individual pathogenic bacteria is various in different growing phase,which has a profound significance for food safety testing and control.In this work,the aptamers against B.cereus in different growing phase was firstly selected based on whole bacterium-SELEX screening approach.Out of 32 candidate aptamers screened,aptamer B15 and B16 showed relatively high binding affinity with an apparent dissociation constant(Kd value)of 16.134.98 nM and 20.675.23 nM,respectively.A label-free impedance based aptasensor was further fabricated based on the screened aptamer,immobilized onto the GCE/Au surface.With the addition of B.cereus,the electron transfer process was prevented and exhibited a significant decrease in the electron-transfer resistance.Under optimal conditions,the fabricated aptasensor can achieve high sensitive detection of B.cereus as low as 10 cfu/mL.A satisfactory recovery of 90.5%–105.3%was achieved for the B.cereus detection in spiked milk samples,exhibiting its prospect in the sensitive detection of B.cereus in daily life.

关 键 词：B.cereus Different growing phase SELEX APTAMER Impedance 

分 类 号：R73[医药卫生—肿瘤]