Prophylactic role of Premna herbacea,a dietary leafy vegetable in managing hepatic steatosis via regulating AMPK/SREBP1/ACC/HMGCR signaling pathway  

作　　者：Simanta Bharadwaj Shalini Gurumayum Devi Pranamika Sarma Barsha Deka Sagar Ramrao Barge Bhaswati Kashyap Yunus Sheikh Prasenjit Manna Jagat C.Borah Narayan Chandra Talukdar 

机构地区：[1]Chemical Biology Lab 1,Institute of Advanced Study in Science and Technology(IASST),Paschim Boragaon,Guwahati,781035,Assam,India [2]Molecular Biology and Biotechnology(MBBT)Department,Cotton University,Guwahati,781001,Assam,India [3]Assam Downtown University,Panikhaiti,Assam,781068,India [4]Biological Science and Technology Division,CSIR-North East Institute of Science and Technology(CSIR-NEIST),Jorhat,785006,Assam,India

出　　处：《Food Bioscience》2022年第4期53-62,共10页食品生物科学（英文）

摘　　要：Non-alcoholic fatty liver disease(NAFLD)includes a broad spectrum of liver dysfunction ranging from hepatic steatosis(intracellular triglyceride accumulation)to steatohepatitis.This study investigated the beneficial effect of Premna herbacea(a dietary leafy vegetable of North East India)and its active constituent in managing hepatic steatosis using high fat diet(HFD)-fed male Wistar rat and hepatocyte(CC1)cell culture models.Administration with methanolic leaf extract of Premna herbacea(PHME)(250 mg/kg body weight,oral gavage,20 weeks)reduced the gain in body weight and rise in serum lipid(triglyceride and total cholesterol)levels and enzymes(alkaline phosphatase and aspartate aminotransferase)responsible for liver dysfunction in high fat diet(HFD)-fed rats.PHME administration further upregulated the phosphorylation of AMPK,ACC,and HMGCR,downregulated SREBP1 signaling pathways of lipid metabolism,and prevented hepatic steatosis in liver tissues of HFD-fed rats.Moreover,studies on cultured hepatocytes revealed that treatment with both crude extract and its ethyl acetate fraction(PHEA)significantly reduced intracellular lipid accumulation in palmitate(PA,0.75 mM)-treated cells.Chemical profiling(HPLC,HRMS,^(1)H and^(13)C NMR)of PHEA demonstrated the presence of Verbascoside,a caffeoyl phenylethanoid glycoside as a major phyto-constituent,which prevented cellular lipid accumulation and regulated the alteration in AMPK/SREBP/ACC/HMGCR signaling pathways of lipid metabolism in PA-treated cells.In conclusion,this study showed the prophylactic role of Premna herbacea and its active molecule,Verbascoside as a potential functional food in ameliorating hepatic steatosis by regulating AMPK/SREBP/ACC/HMGCR signaling cascade.

关 键 词：Intracellular lipid accumulation Hepatic steatosis Premna herbacea Dietary leafy vegetable of North East India VERBASCOSIDE AMPK/SREBP/PPARαsignaling pathways Hepato-protection 

分 类 号：TS201.4[轻工技术与工程—食品科学] R575[轻工技术与工程—食品科学与工程]