基于网络药理学探究桑黄多酚的体外抗肿瘤作用及其机制  

In Vitro Anti-tumor Effect and Its Mechanism of Polyphenols from Sanghuangporus vaninii Based on Network Pharmacology

作　　者：王雨阳 刘朋 张忠[1] 陈万超[1] 吴迪[1] 李文[1] 杨焱[1] WANG Yu-yang;LIU Peng;ZHANG Zhong;CHEN Wan-chao;WU Di;LI Wen;YANG Yan(Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,National Engineering Research Center of Edible Fungi,Key Laboratory of Edible Fungi Resources and Utilization(South),Ministry of Agriculture,Shanghai 201403)

机构地区：[1]上海市农业科学院食用菌研究所农业农村部南方食用菌资源利用重点实验室国家食用菌工程技术研究中心国家食用菌加工技术研发中心,上海201403

出　　处：《生物技术通报》2024年第11期68-77,共10页Biotechnology Bulletin

基　　金：上海市科委农业领域项目(22N51900100);上海领军人才队伍建设专项资金项目。

摘　　要：【目的】分离、筛选瓦尼桑黄(Sanghuangporus vaninii)子实体中具有良好抗肿瘤活性的多酚组分,并对其抗肿瘤活性和潜在作用机制进行解析。【方法】采用超声辅助提取法提取乙酸乙酯部位的组分,通过对肝癌细胞HepG-2的抑制活性筛选出具有良好抗肿瘤作用的多酚组分,基于超高效液相色谱-四极杆飞行时间质谱(UPLC-Q-TOF-MS)技术鉴定其主要成分,利用网络药理学策略阐释其潜在抗肿瘤作用机制。【结果】分离制备获得了5种(SVa-SVe)多酚组分,其中SVe多酚组分表现出独特的抗肿瘤活性,100μg/mL浓度下对HepG-2细胞抑制率为76.54%。SVe可以显著地促进HepG-2细胞凋亡和诱导其细胞周期阻滞,并呈现剂量依赖性。成分分析显示,SVe主要由31种化合物组成,基于网络药理学分析表明,SVe中的osmundacetone、hispolon、phellibaumin A、davallialactone等关键化合物通过与多个靶点蛋白作用(STAT3、mTOR、VEGFA、SRC、ERBB2和HSP90AA),发挥抗肿瘤活性。【结论】来源于桑黄的多酚提取物SVe通过诱导细胞凋亡和细胞周期阻滞对HepG-2细胞具有独特的抑制活性,其中的多酚化合物通过多靶点发挥作用。【Objective】The polyphenolic components with excellent anti-cancer activity from the fruiting body of Sanghuangporus vaninii were isolated and screened,and their anti-cancer activity and potential mechanisms of action were analyzed.【Method】Ultrasoundassisted extraction was used to extract components from the ethyl acetate fraction.Polyphenolic components with excellent anti-cancer effects were screened for their inhibitory activity on liver cancer cell HepG-2.The main components were identified using ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS)technology,and the potential mechanism of anticancer was elucidated using network pharmacology strategies.【Result】Five polyphenolic components(SVa-SVe)were isolated and prepared,among which SVe polyphenolic component demonstrated unique anti-cancer activity,with an inhibition rate of 76.54%on HepG-2 cells at a concentration of 100μg/mL.SVe significantly promoted apoptosis and induced cell cycle arrest in HepG-2 cells,showing a dose-dependent effect.Ingredient analysis showed that SVe was mainly composed of 31 compounds.Based on network pharmacology analysis,key compounds such as osmundacetone, hispolo, phellibaumin A, davallialactone in SVe exerted anti-cancer activity by interacting with multiple target proteins(STAT3, mTOR, VEGFA, SRC, ERBB2, and HSP90AA).【Conclusion】The extract SVe derived from S. vaninii possessed unique inhibitoryactivity on HepG-2 cells by inducing apoptosis and cell cycle arrest, which was related to the presence of polyphenolic substances.

关 键 词：瓦尼桑黄 多酚 肝癌 网络药理学 

分 类 号：R285[医药卫生—中药学]