虾青素对α-鹅膏毒肽致小鼠急性肝损伤保护作用的研究  

Protective effect of astaxanthin on acute liver injury induced byα-amanitin in mice

作　　者：罗永平 钟加菊 姚群梅 耿正祥 陈冲贵 余成敏 Luo Yongping;Zhong Jiaju;Yao Qunmei;Geng Zhengxiang;Chen Chonggui;Yu Chengmin(Clinical College,Dali University,Dali 671000,China;Emergency Department,The People's Hospital of Chuxiong Yi Autonomous Prefecture,Chuxiong 675000,China;Emergency Department,The First Affiliated Hospital of Dali University,Dali 671000,China;College of Pharmacy,Dali University,Dali 671000,China)

机构地区：[1]大理大学临床医学院,大理671000 [2]楚雄彝族自治州人民医院急诊科,楚雄675000 [3]大理大学第一附属医院急诊科,大理671000 [4]大理大学药学院,大理671000

出　　处：《中华劳动卫生职业病杂志》2024年第11期801-806,共6页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：云南省重大科技专项计划(2018ZF009);科技人才与平台计划(院士专家工作站)(202305AF150066)。

摘　　要：目的探究虾青素对α-鹅膏毒肽致小鼠急性肝损伤的保护作用。方法于2023年6月,选择42只健康SPF级雄性昆明小鼠,采用随机数字表法将小鼠分为空白对照组、模型(0.45 mg/kgα-鹅膏毒肽)组、橄榄油(10 ml/kg橄榄油)组、低剂量(20 mg/kg)虾青素组、中剂量(40 mg/kg)虾青素组、高剂量(80 mg/kg)虾青素组以及水飞蓟宾(20 mg/kg)组,每组6只。空白对照组小鼠通过腹腔注射10 ml/kg生理盐水,其余组小鼠注射α-鹅膏毒肽;之后空白对照组和模型组灌胃10 ml/kg生理盐水,橄榄油组和虾青素组按剂量灌胃橄榄油和虾青素,水飞蓟宾组按剂量注射水飞蓟宾,每12 h给药1次,共给药4次。60 h后处死小鼠,称量肝脏质量,计算肝脏指数;检测小鼠血清中天冬氨酸氨基转移酶(aspartate aminotransferase,AST)、丙氨酸氨基转移酶(alanine aminotransferase,ALT)含量,以及肝脏组织中超氧化物歧化酶(superoxide dismutase,SOD)、还原型谷胱甘肽(reduced glutathione,GSH)、过氧化氢酶(catalase,CAT)、丙二醛(malondialdehyde,MDA)含量。采用单因素方差分析比较各组小鼠指标的差异,两两比较采用Dunnett-t检验。结果空白对照组小鼠毛色光滑、精神良好、行为正常,其余组小鼠均出现不同程度的行动迟缓、饮食下降,各组小鼠均未出现死亡。模型组小鼠体质量[(26.67±1.51)g]及肝脏质量[(1.23±0.14)g]均明显低于空白对照组[(33.50±2.43)g和(1.87±0.16)g],差异均有统计学意义(P<0.05);低、中、高剂量虾青素组和水飞蓟宾组小鼠肝脏指数[(5.39±0.32)%、(5.83±0.30)%、(5.75±0.24)%和(5.78±0.16)%]均明显高于模型组[(4.61±0.12)%],差异均有统计学意义(P<0.05)。模型组小鼠血清中ALT及AST含量[(153.04±13.96)U/L和(59.08±4.03)U/L]均明显高于空白对照组[(13.77±1.29)U/L和(10.21±0.35)U/L],差异均有统计学意义(P<0.05)。模型组小鼠肝脏组织中CAT、GSH和SOD含量[(9.40±2.23)U/mgprot、(3.09±0.26)μmol/gprot和(48.94±ObjectiveTo explore the protective effect of astaxanthin on acute liver injury induced byα-amanitin in mice.MethodsIn June 2023,42 healthy SPF male Kunming mice were selected.The mice were divided into blank control group,model(0.45 mg/kgα-amanitin)group,olive oil(10 ml/kg olive oil)group,low dose(20 mg/kg)astaxanthin group,medium dose(40 mg/kg)astaxanthin group,high dose(80 mg/kg)astaxanthin group and silybin(20 mg/kg)group by random number table method.Each group had 6 animals.Mice in the blank control group were intraperitoneally injected with 10 ml/kg normal saline,and mice in the other group were injected withα-amanitin.After that,the blank control group and model group were infused with 10 ml/kg normal saline,olive oil group and astaxanthin groups were given olive oil and astaxanthin according to dose by gavage,and silybin group was injected with silybin by dose.The drug was administered once every 12 h for a total of 4 doses.After 60 h,the mice were killed,the liver weight was weighed,and the liver index was calculated.The contents of aspartate aminotransferase(AST)and alanine aminotransferase(ALT)in serum of mice were detected,and the contents of superoxide dismutase(SOD),reduced glutathione(GSH),catalase(CAT),malondialdehyde(MDA)in liver tissues were also detected.One-way analysis of variance(ANOVA)was used to compare the difference of indexes among each group,and pairwise comparison was performed by Dunnett-t test.ResultsThe mice in the blank control group had smooth hair color,good spirit and normal behavior,while the mice in the other groups showed varying degrees of retardation and decreased diet,and no death occurred in each group.Body mass[(26.67±1.51)g]and liver mass[(1.23±0.14)g]in model group were significantly lower than those in blank control group[(33.50±2.43)g and(1.87±0.16)g],and the differences were statistically significant(P<0.05).The liver index[(5.39±0.32)%,(5.83±0.30)%,(5.75±0.24)%and(5.78±0.16)%]in low,medium and high dose astaxanthin groups and silybin group were signific

关 键 词：小鼠 虾青素 α-鹅膏毒肽 水飞蓟宾 肝损伤 氧化应激 转氨酶类 超氧化物歧化酶(SOD) 还原型谷胱甘肽(GSH) 过氧化氢酶(CAT) 丙二醛(MDA) 蕈中毒 

分 类 号：R575[医药卫生—消化系统]