低压静电场处理对樱桃番茄采后品质及 线粒体氧化还原代谢的影响  

Effect of Low-Voltage Electrostatic Field Treatment on Postharvest Quality and Mitochondrial Redox Metabolism of Cherry Tomatoes

作　　者：牛培荣 马存福 魏晓博 马立虎 刘慧燕 方海田[1] NIU Peirong;MA Cunfu;WEI Xiaobo;MA Lihu;LIU Huiyan;FANG Haitian(College of Food Science and Engineering,Ningxia University,Yinchuan 750021,China)

机构地区：[1]宁夏大学食品科学与工程学院,宁夏银川750021

出　　处：《食品科学技术学报》2024年第6期91-101,共11页Journal of Food Science and Technology

基　　金：宁夏自然科学基金项目(2022AAC03020);宁夏回族自治区青年拔尖人才工程项目(022004000010);宁夏大学贺兰山学者项目(2020)。

摘　　要：低压静电场技术是一种可以延长果蔬保鲜期的技术,为探究其对果蔬采后品质的影响,以樱桃番茄为材料,研究低压静电场(200、300 mV)处理对樱桃番茄在(4±1)℃贮藏期间品质、活性氧代谢和线粒体氧化还原代谢的影响。结果表明:与对照组相比,低压静电场处理降低了樱桃番茄呼吸速率,延缓了失重率的上升和可溶性固形物、酸度的下降,保持了樱桃番茄的硬度;同时,低压静电场处理显著提高了贮藏期间樱桃番茄的过氧化物酶、过氧化氢酶和超氧化物歧化酶的活力以及抗坏血酸的含量(P<0.05),进而抑制过氧化氢和超氧阴离子自由基等活性氧的产生和积累。进一步分析发现,低压静电场处理还可以抑制樱桃番茄线粒体细胞色素c/a的降低,提高一氧化氮含量,以维持线粒体氧化还原状态;同时,可提高线粒体NAD+含量、降低NADH含量,提高NAD+/NADH比例,使线粒体氧化还原电势处于较高水平。低压静电场处理可抑制樱桃番茄的活性氧产生和维持线粒体氧化还原的平衡状态进而保持樱桃番茄贮藏品质。Low-voltage electrostatic field technology is a technique that can prolong the shelf life of fruits and vegetables.To explore its impact on the postharvest quality of fruits and vegetables,the effects of low-voltage electrostatic field(200 mV and 300 mV)treatment on the quality,reactive oxygen metabolism and mitochondrial redox metabolism of cherry tomatoes during storage at(4±1)℃were investigated using cherry tomatoes as materials.The results showed that compared with the control group,the low-voltage electrostatic field treatment reduced the respiration rate,delayed the increase of weight loss rate and the decrease of soluble solids and acidity,and maintained the hardness of cherry tomatoes.At the same time,the low-voltage electrostatic field treatment significantly increased the activities of peroxidase,catalase,and superoxide dismutase as well as the content of ascorbic acid in cherry tomatoes during storage(P<0.05),and in turn inhibited the production and accumulation of reactive oxygen species,such as hydrogen peroxide and superoxide radical.Further analysis revealed that low-voltage electrostatic field treatment also inhibited the decrease of mitochondrial cytochrome c/a and increased nitric oxide content in cherry tomatoes to maintain the mitochondrial redox state.Meanwhile,it increased mitochondrial NAD+content and decreased NADH content,increased the NAD+/NADH ratio,and kept the mitochondrial redox potential at a high level.Low-voltage electrostatic field treatment could inhibit the generation of reactive oxygen species and maintain the balance of mitochondrial redox state in cherry tomatoes,thus maintaining their storage quality.

关 键 词：低压静电场 樱桃番茄 采后品质 活性氧代谢 线粒体氧化还原 

分 类 号：TS255.3[轻工技术与工程—农产品加工及贮藏工程]