马齿苋多酚的半纤维素酶提取工艺优化及在蛋鸡中应用研究  

Optimization of Hemicellulase Extraction Process for Polyphenols from Portulaca oleracea and Application Research in Laying Hens

作　　者：李清筱[1] LI Qingxiao(Henan Industry and Trade Vocational College,Henan Zhengzhou 451191,China)

机构地区：[1]河南工业贸易职业学院,河南郑州451191

出　　处：《饲料工业》2024年第23期70-77,共8页Feed Industry

摘　　要：研究旨在优化马齿苋多酚的提取工艺并探讨马齿苋多酚在蛋鸡上的应用效果,以半纤维素酶添加量、酶解时间、酶解温度进行单因素试验,并按照单因素试验结果设计响应面优化试验,然后将提取的多酚化合物分别按0.1%、0.2%、0.3%、0.4%添加在蛋鸡基础日粮中,研究马齿苋多酚对蛋鸡生产性能和蛋黄抗氧化能力的影响。结果显示:马齿苋多酚的半纤维素酶提取工艺响应面法优化后最优提取工艺为:酶解温度45℃、酶解时间6 h、酶添加量1.7%,在此条件下多酚得率为8.77%;随马齿苋多酚化合物添加比例的增加,蛋鸡的平均日采食量、产蛋率、平均蛋重、蛋黄的抗氧化能力逐渐增加,而料蛋比逐渐减小。结果表明,用半纤维素酶优化提取马齿苋中的多酚化合物,多酚得率为8.77%;将提取的多酚化合物添加到蛋鸡日粮中,可节省饲料,改善蛋鸡的生产性能和鸡蛋的抗氧化能力。The study was intended to optimize the extraction process of purslane polyphenols and investigate their application effects in laying hens.A single-factor test was conducted with hemicellulase,enzymolysis time,and enzymolysis temperature.Based on the results of the single-factor test,a response surface method was designed to optimize the experiment.The extracted polyphenolic compounds were added to the laying hens'basal diet at levels of 0.1%,0.2%,0.3%,and 0.4%to study the effects of purslane polyphenols on the production perfor‐mance of laying hens and the antioxidant capacity of egg yolk.The results showed that the optimal extraction process of purslane polyphenols using the hemicellulase response surface method was an enzymolysis temperature of 45℃,enzymolysis time of 6 hours,and an enzyme addition amount of 1.7%,under which the polyphenol yield was 8.77%.As the proportion of purslane polyphenolic compounds increased,the daily feed intake,egg production rate,average egg weight,and yolk antioxidant capacity of laying hens gradually increased,while the feed-to-egg ratio gradually decreased.The results showed that optimizing the extraction of polyphenolic compounds from purslane using hemicellulase and adding them to the diet of laying hens can save feed,improve production performance,and enhance the antioxidant capacity of eggs.

关 键 词：马齿苋多酚 半纤维素酶 多酚化合物得率 蛋鸡生产性能 抗氧化能力 

分 类 号：S816.7[农业科学—饲料科学]