Photothermal probiotics delivery of photodynamic agents for enhanced immunogenic cell death within hypoxia center of solid tumors  

作　　者：Wen Luo Shiyu Du Yateng Jiang Bangshun He Jun Tang Jingjing Yang Yujun Song 

机构地区：[1]Jiangsu Key Laboratory of Artificial Functional Materials,State Key Laboratory of Analytical Chemistry for Life Science,National Laboratory of Solid State Microstructures,College of Engineering and Applied Sciences,Nanjing University,Nanjing 210023,China [2]Department of Laboratory Medicine,Nanjing First Hospital,Nanjing Medical University,Nanjing 210006,China [3]Department of Anesthesiology,Jinling Hospital,Medical School of Nanjing University,Nanjing 210000,China [4]Department of Biochemistry and Molecular Biology,School of Medicine&Holistic Integrative Medicine,Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,Nanjing University of Chinese Medicine,Nanjing 210023,China

出　　处：《Nano Research》2025年第1期642-652,共11页纳米研究（英文版）

基　　金：supported by the National Key R&D Program of China(No.2019YFA0709200);the National Natural Science Foundation of China(Nos.22477056 and 82472379);the Fundamental Research Funds for Central Universities,the Natural Science Foundation of Jiangsu Province(No.BK20200336);the Key Research and Development Program of Jiangsu Province(No.BE2021373);the Program for Innovative Talents and Entrepreneur in Jiangsu,and the Postgraduate Research&Practice Innovation Program of Jiangsu Province(No.KYCV20_0036).

摘　　要：Resulting from harsh hypoxic environmental conditions,central cells in the core of solid tumors are usually more aggressive and malignant with a less stable genome.Therefore,therapeutic agents with improved penetration for the activation of immunity in tumor centers exhibit promising potential in immunotherapies.Here,polydopamine-coated Escherichia coli Nissle 1917(EcN)bearing chlorin e6(Ce6)-loaded and polyethyleneimine(PEI)-coated hollow manganese dioxide(shorted as EP-ChP)are applied for enhanced immunotherapy in deep tumors.After accumulation in tumor center through hypoxia targeting,manganese dioxide is degradated under the tumor microenvironment with released Ce6 and thus generates reactive oxygen species(ROS)upon 660 nm laser irradiation,which can further lower thermalresistance of cancer cells via HSP90αdownregulation.Owing to that,heating induced by polydopamine upon 808 nm laser irradiation can achieve effective tumor ablation.Phototherapy upon dual laser induces enhanced immunogenic cell death,while bacterial infections in tumor tissues also trigger innate immunity.This bacteria-based approach provides enhanced antitumor immune responses in deep tumors with great potential in the reshaping of immunosuppression tumor microenvironment.

关 键 词：BACTERIA phototherapies IMMUNOTHERAPY hypoxia targeting tumor penetration 

分 类 号：R730.51[医药卫生—肿瘤]