不同来源SSR引物对刺梨种群遗传差异性分析  

Genetic Differences of Rosa roxburghii Tratt.Using SSR Primers from Different Sources

作　　者：刘粉粉 黄秦军[1] 高铖铖 刘进 欧国腾 莫亮团 LIU Fen-fen;HUANG Qin-jun;GAO Cheng-cheng;LIU Jin;OU Guo-teng;MO Liang-tuan(Research Institute of Forestry Chinese Academy of Forestry,Beijing 100091,China;Qiannan Forestry Bureau of Guizhou Province,Duyun 558099,Guizhou,China)

机构地区：[1]中国林业科学研究院林业研究所,北京100091 [2]贵州省黔南州林业局,贵州都匀558099

出　　处：《林业科学研究》2024年第6期171-179,共9页Forest Research

基　　金：中央级公益性科研院所基本科研业务费专项资金(CAFYBB2020ZC001)。

摘　　要：[目的]研究刺梨EST-SSR与近缘物种萨蔓莎月季的EST-SSR对刺梨种群遗传分析产生的差异,为刺梨遗传多样和遗传结构研究提供不同来源SSR标记位点。[方法]采用6对萨蔓莎月季EST-SSR引物和6对刺梨EST-SSR引物对261个野生刺梨样本进行基因分型,利用GenAlex软件估算群体遗传多样性参数、遗传分化系数和基因流,并进行AMOVA分子方差分析,采用SPSS Statistics软件进行方差分析和相关性分析,NTSYS软件计算遗传相似度并按照UPGMA方法进行聚类。[结果]12对SSR引物共扩增出95条带,其中萨蔓莎月季SSR引物扩增出53条带,刺梨SSR引物扩增出42条带。基于萨蔓莎月季SSR引物估算出的平均有效等位基因数目、期望杂合度、多态性信息含量和香浓信息指数分别为2.206、0.477、0.459和0.862,比刺梨SSR引物估算出的相应指标分别低了1.242、0.205、0.215和0.451。遗传分化结果显示,基于月季SSR和刺梨SSR估算的遗传分化系数Fst分别为0.128和0.062,均表明群体属于低等遗传分化水平。基于两种SSR标记的群体AMOVA分析结果基本相同,遗传变异主要来源于群体内。遗传距离相关性结果显示,两种来源的SSR得到的结果无显著相关性,而聚类结果相似。[结论]萨蔓莎月季SSR与刺梨SSR在进行遗传多样性和遗传距离分析时存在一定的差异,但差异并不显著;遗传分化和AMOVA分析得到的结果极为相似,表明近缘物种萨蔓莎月季的SSR在刺梨中通用性比较好,为后续刺梨种质资源收集、品种鉴定和指纹图谱构建提供了标记位点,为蔷薇科植物遗传多样性分析提供了新的思路。[Objective]The objective of this was to study the differences in the genetic analysis of R.roxburghii population between the EST-SSR of R.roxburghii and its related species Rosa chinensis,in order to provide SSR markers from different sources for the study of R.roxburghii genetic diversity and structure.[Method]Six pairs of R.chinensis EST-SSR primers and six pairs of R.roxburghii EST-SSR primers were used to genotype 261 wild R.roxburghii samples.GenAlex software was used to estimate population genetic diversity parameters,genetic differentiation coefficients,and gene flow,and AMOVA molecular variance analysis was performed.Variance analysis and correlation analysis were performed using SPSS software,and genetic similarity and cluster were analyzed according to UPGMA method through NTSYS software.[Result]A total of 95 bands were amplified by 12 pairs of SSR primers,of which 53 bands were amplified by R.chinensis SSR primers and 42 bands were amplified by R.roxburghii SSR primers.The average expected number of alleles,expected heterozygosity,polymorphism information content,and Shannon`s information index estimated based on Rosa chinensis Jacq.SSR primers were 2.206,0.477,0.459,and 0.862,respectively.They were 1.242,0.205,0.215,and 0.451 lower than the corresponding indicators estimated based on Rosa roxburghii Tratt.SSR primers,respectively.The genetic differentiation results showed that the estimated genetic differentiation coefficients(FST)based on rose SSR and R.roxburghii SSR were 0.128 and 0.062,respectively,indicating that the population belongs to a lower level of genetic differentiation.The results of population AMOVA analysis based on two SSR markers were very similar,and genetic variation mainly came from within the population.The genetic distance correlation results showed that the results obtained from both sources of SSR were significantly correlated with the results obtained from mixed SSR,and the clustering results were also basically consistent.[Conclusion]There are certain differences in genetic

关 键 词：刺梨 萨蔓莎月季 EST-SSR 遗传多样性 相关性分析 

分 类 号：S792.11[农业科学—林木遗传育种]