Multidimensional screening of Astragalus membranaceus small molecules to mitigate carbon ion radiation-induced bystander effects  

作　　者：Liying Zhang Yiming Zhang Yangyang Li Qiyang Li Shangzu Zhang Zhiming Miao Jinpeng He Ting Zhou Gengqiang Yang Xin Wang Jufang Wang Yongqi Liu 

机构地区：[1]Provincial-Level Key Laboratory for Molecular Medicine of Major Diseases and the Prevention and Treatment with Traditional Chinese Medicine Research in Gansu Colleges and Universities,Gansu University of Chinese Medicine,Lanzhou,73000,China [2]Gansu Institute of Cardiovascular Diseases,Lanzhou,730000,China [3]Key Laboratory of Space Radiobiology of Gansu Province&Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences,Institute of Modern Physics,Chinese Academy of Sciences,Lanzhou,730000,China

出　　处：《Journal of Pharmaceutical Analysis》2024年第10期1531-1534,共4页药物分析学报（英文版）

基　　金：This work was supported by the National Natural Science Foundation of China(Grant Nos.:82260882 and 82004094).

摘　　要：Existing studies have shown that Astragalus membranaceus(AM)and its active ingredients astragalus polysaccharides,oninon,and astragalus methyl glycosides can attenuate X-ray radiation-induced injury.However,there are no studies on how isoliquiritigenin(ISL)attenuate the bystander effect of bone marrow mesenchymal stem cells(BMSCs)induced by carbon ion radiation therapy for lung cancer.This study aimed to investigate the AM-derived small molecule ISL to enhance radiotherapy sensitivity by attenuating the carbon ion radiation-induced bystander effect(RIBE)in BMSCs to elucidate its mechanism of action.In this study,we established a C57BL/6 mouse lung cancer transplantation tumor model in vivo and a co-culture model of A549 cells and BMSCs in vitro,and the models were successfully treated with carbon ions.In further work,we used flow cytometry,immunofluorescence,Western blot,enzyme-linked immunosorbent assay(ELISA),inhibitor,short hairpin RNA(shRNA),Cell Counting Kit-8(CCK-8),and other methods to illustrate the mechanism.In the next experiments,we found that ISL combined with carbon ion radiotherapy had a significant anti-tumor effect and protected BMSCs from radiation damage.The aim of this study was to investigate the potential of ISL in enhancing the sensitivity of lung cancer cells to radiotherapy and attenuating RIBE in both in vitro and in vivo settings.Traditional Chinese medicine combined with radiation therapy is a promising and innovative treatment for non-small cell lung cancer.These results establish a theoretical foundation for further clinical development of ISL as a potential radiosensitizer option.

关 键 词：RADIOTHERAPY BMSCS HIF-1α Astragalus membranaceus ISOLIQUIRITIGENIN Non-small cell lung 

分 类 号：R285[医药卫生—中药学]