植物乳杆菌YHG1-155发酵沙棘汁促糖苷类多酚生物转化  

The Biotransformation of Glycoside Polyphenols in Sea Buckthorn Juice by Lactobacillus plantarum YHG1-155 Fermented

作　　者：陈彦辉 李亚凤 李璞钰 庄姣 高岩 剧柠[1] Chen Yanhui;Li Yafeng;Li Puyu;Zhuang Jiao;Gao Yan;Ju Ning(College of Food Science and Engineering,Ningxia University,Yinchuan 750021)

机构地区：[1]宁夏大学食品科学与工程学院,银川750021

出　　处：《中国食品学报》2024年第11期289-299,共11页Journal of Chinese Institute Of Food Science and Technology

基　　金：宁夏回族自治区自然科学基金项目(2022AAC 03023);宁夏回族自治区重点研发计划项目(2021BEF02022)。

摘　　要：发酵有助于糖苷类多酚向苷元类多酚转化,在提高生物利用度的同时,提升产品的风味。本研究通过植物乳杆菌YHG1-155发酵沙棘汁,分析其体外抗氧化活性的变化,探究发酵过程中多酚化合物的生物转化。结果显示,发酵提高了沙棘汁的活菌数、游离酚含量、总酚含量和抗氧化活性。采用超高效液相色谱-串联质谱(UPLC-MS/MS)技术从发酵沙棘汁中共鉴定出116种差异代谢物。其中,Y2 v.s.Y4组,Y4 v.s.Y8组以及Y2 v.s.Y8组分别鉴定出67种(53种上调和14种下调),64种(32种上调和32种下调)和116种(74种上调和42种下调)差异代谢物。KEGG通路富集分析表明,Y2 v.s.Y4组的18种以及Y4 v.s.Y8组的13种差异代谢物主要参与3条生物合成途径,即次生代谢物的生物合成途径、类黄酮的生物合成途径以及黄酮和黄酮醇的生物合成途径。其中,次生代谢物的生物合成途径是沙棘多酚转化的重要通路,多酚糖苷可能主要通过该途径向苷元转化。本研究从代谢组学角度揭示植物乳杆菌YHG1-155发酵对沙棘多酚糖苷的生物转化的影响,为沙棘精深加工提供理论参考。Fermentation,contributing to convert polyphenol glycosides to their respective aglycones,could improve their bioavailability as well as flavor of the product.This study performed Lactobacillus plantarum YHG1-155 fermentation of sea buckthorn juice(SBJ)to analysis the changes of its antioxidant activity in vitro and explore the biotransformation process of polyphenols in the fermentation.The results showed that L.plantarum fermentation increased the viable cell count,free phenolic content,total phenolic content,and antioxidant activity of SBJ.In total,116 significantly different metabolites were identified in L.plantarum fermented SBJ using ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).Among them,67(53 upregulated and 14 downregulated),64(32 upregulated and 32 downregulated),and 116(74 upregulated and 42 downregulated)kinds of differential metabolites were identified between the groups Y2 and Y4,Y4 and Y8,and Y2 and Y8.Furthermore,KEGG pathway enrichment analysis showed that 18 kinds of differential metabolites between Y4 and Y2 and 13 kinds of differential metabolites between Y8 and Y4 were mainly involved in three pathways,namely the biosynthesis of secondary metabolites,flavonoid,flavone and flavonol biosynthesis pathways.Among them,the biosynthesis pathway of secondary metabolites was an important pathway for polyphenol biotransformation,and polyphenol glycosides might mainly be converted to their respective aglycones through this pathway.Our findings revealed the biotransformation of polyphenol glycosides of SBJ by L.plantarum YHG1-155 fermentation,providing a theoretical reference for the deep processing of hippophae rhamnoides.

关 键 词：发酵沙棘汁 植物乳杆菌YHG1-155 酚类化合物 代谢组学 

分 类 号：TS255.44[轻工技术与工程—农产品加工及贮藏工程] TS201.3[轻工技术与工程—食品科学与工程]