Flavonoid rich extract of Trigonella foenum-graecum leaves ameliorate liver fibrosis  

作　　者：Faiza Shams Ayesha Azeem Ammara Shams Abdul Tawab Sidra Rehman Somayya Tariq Noreen Latief Bushra Ijaz 

机构地区：[1]Centre of Excellence in Molecular Biology,University of the Punjab,Lahore,Pakistan [2]National Institute for Biotechnology and Genetic Engineering,Jhang Road,Faisalabad,Pakistan [3]Department of Biosciences,University of COMSATS,Islamabad,Pakistan

出　　处：《Food Bioscience》2022年第6期444-455,共12页食品生物科学（英文）

基　　金：the Higher Education Commission Pakistan for providing a research grant(NRPU-8719)to conduct the research.

摘　　要：Liver fibrosis is a leading cause of mortality and morbidity globally.For decades,plant secondary metabolites such as flavonoids and saponins have attained much consideration due to their safer therapeutic potential against chronic liver diseases.Trigonella foenum-graecum(Common name“Fenugreek”)is an annual herb used as a spice to increase the taste and aroma of food.Its fresh leaves are used as food and are rich in flavonoids.In the current study antifibrotic potential of Trigonella foenum-graecum flavonoid-rich leaf extract(TF)was evaluated in CCl4-induced hepatic fibrosis in mice.The ALT,AST,MDA,CAT,and GSH analysis illustrated that the treatment group significantly reverses CCl4-induced liver damage.The TF extract was found to be rich in quercetin and kaempferol derivatives analyzed through ESI-MS/MS.After four weeks of the TF treatment,hepatocyte architecture improved and collagen deposition in mice liver tissues reduced considerably.The expression of fibrotic markers,including COL-IA,α-SMA,PDGF,and TIMP1,was downregulated,and MMP3 was upregulated significantly at mRNA and protein levels.Interestingly,TF significantly downregulated NF-κB expression and TGF-β/Smad signaling pathway and upregulated miR-146a and miR-29b.Taking together,these results demonstrated that Trigonella foenum-graecum flavonoid-rich leaf extract ameliorated liver inflammatory and fibrogenic responses through miR-146a/miR-29b/TGF-β/Smad axis.

关 键 词：Liver fibrosis FLAVONOIDS Trigonella foenum-Graecum Inflammation FENUGREEK TGF-BETA 

分 类 号：TS201.4[轻工技术与工程—食品科学]