Chemical characterization UPLC-ESI-QToF-MSE,antibacterial and antibiofilm potential of Sarcomphalus joazeiro(MART.)Hauenschild  

作　　者：Nara J.S.Araújo Ana Raquel P.Silva Maria S.Costa Thiago S.Freitas José M.Barbosa Filho Yedda M.L.S.Matos Maria Flaviana B.Morais-Braga Francisco N.Pereira Junior C.A.P.Silva Erlânio O.Souza Paulo R.V.Ribeiro Bruna Caroline Gonçalves Vasconcelos de Lacerda Edlane Martins de Andrade Henrique D.M.Coutinho Jacqueline C.Andrade-Pinheiro 

机构地区：[1]Programa de pós-graduação em Ciências da Saúde,Universidade Federal do Cariri,UFCA,Divino Salvador,284,CEP,63180-000,Barbalha,CE,Brazil [2]Programa de pós-graduação em Biotecnologia,Universidade Estadual do Ceará,UECE,Av.Dr Munguba,1700,CEP,60714-903,Fortaleza,CE,Brazil [3]Departamento de Química Biológica,Universidade Regional do Cariri,URCA,Cel Antônio Luis,1161,CEP,63105-000,Crato,CE,Brazil [4]Laboratório de Tecnologia Farmacêutica,Universidade Federal da Paraíba,UFPB,Cidade Universitária,5009,CEP,58051-970,João Pessoa,PB,Brazil [5]Laboratório de Bioensaios – LABIO,Universidade Federal do Cariri – UFCA,R.Olegário Emidio de Araujo,s/n,CEP,63260-000,Brejo Santo,CE,Brazil [6]Faculdade de Tecnologia do Cariri,FATEC,Amália Xavier de Oliveiro,CEP,63040-000,Juazeiro do Norte,CE,Brazil [7]Embrapa Agroindústria Tropical,R.Pernambuco,2270 - Pici,60511-110,Fortaleza,CE,Brazil [8]CECAPE College.Av.Padre Cícero,3917 - São José,Juazeiro do Norte,CE,63024-015,Brazil

出　　处：《Food Bioscience》2022年第6期1249-1259,共11页食品生物科学（英文）

基　　金：Microbiology and Molecular Biology Laboratory of the Regional University of Cariri(URCA);Project:Natural Projects as a Control Strategy against Bacterial Biofilms Public Notice/Call:Research Productivity Grant,Stimulation of Internalization and Technological Innovation-BPI 02/2022,number BP4-0172-00215.01.00/20 SPU n:09685010/2020.

摘　　要：The present study aims to demonstrate the chemical composition of the ethanolic extract of Sarcomphalus joazeiro bark and to evaluate its antibacterial and antibiofilm activity.For that,the ethanolic extract of the stem bark was prepared,and chemical characterization was carried out in UPLC-ESI-QToF-MSE.Minimum inhibitory concentration and antibiotic modification test were performed by the microdilution method.Formation and antiformation assays were evaluated by the crystal violet method.In the chemical characterization,the presence of 24 peaks was observed.Antibacterial activity showed minimum inhibitory concentration≥2048μg/mL.In the evaluations of the potentiating activity,synergism was the most evident.In the formation analysis,it was observed that most strains showed moderate biofilm formation.It was concluded that saponin derivatives are the major secondary metabolites in the analyzed extract and that it presents relevant synergism with the antibiotics studied and a significant ability to inhibit bacterial biofilm formation.

关 键 词：BIOFILM SAPONINS Synergism Inhibition 

分 类 号：TS201.3[轻工技术与工程—食品科学]