Testing of a new high voltage electrical discharge generator prototype at high frequencies to assist anthocyanin extraction from blueberries  

作　　者：R.Díaz-Álvarez D.Carullo G.Pataro G.Ferrari L.Segura-Ponce 

机构地区：[1]Department of Food Engineering,Universidad del Bío-Bío,P.O.Box 447,Chillán,Chile [2]Department of Industrial Engineering,University of Salerno,via Giovanni Paolo II,132,84084,Fisciano “SA”,Italy [3]ProdAl Scarl –– University of Salerno,via Ponte don Melillo,84084,Fisciano “SA”,Italy

出　　处：《Food Bioscience》2022年第6期1296-1306,共11页食品生物科学（英文）

基　　金：supported by the Universidad del Bío-Bío[Scholarship fund for postgraduate research],the INNOVA BIOBIO[16IP-65192 project].

摘　　要：Traditional extraction methods are based on high-temperature maceration with organic solvents,which are dangerous for human health.A viable alternative to overcome the issues associated with conventional extraction is to increase cell tissue permeability by applying high voltage electrical discharge(HVED)treatments.The objective of this work was to validate the electroporation of blueberry plant cells using a new HVED generator prototype at a high frequency,investigate the effect,intensity,and duration of the applied voltage,and recover anthocyanins from its electroporated cells.The electroporation level of the HVED-treated blueberries was measured qualitatively by transmission electron microscopy(TEM)analysis.Meanwhile,it was quantitatively measured by the cell permeabilization index(Zp)and anthocyanin extraction level.Results of the micrographs(TEM)showed electroporation in all treatments in which Zp was 0.24 when applying a 2 kV treatment for 2 s,whereas a 3-fold increase in tissue damage was revealed with the most powerful treatment(10 kV voltage,30 s).In addition,anthocyanin values ranged from 83.09±1.20(control)to 136.82±0.84(HVED),which was 64.66%higher.The HVED treatment can increase mass transfer rates during conventional extraction processes.It should be noted that the validated prototype required a low specific energy requirement(31–204 kJ/kg)for proper tissue electropermeabilization.In conclusion,we demonstrated the capability of the developed HVED prototype to boost mass transfer phenomena and thus potentially increase its adaptability to assist dissimilar industrial processes or waste(e.g.,peels and seeds)such as freeze-drying operations.

关 键 词：Green extraction Cell permeability High voltage electrical discharge Anthocyanins BLUEBERRY 

分 类 号：TS201.3[轻工技术与工程—食品科学]