Effects of exopolysaccharides from Antrodia cinnamomea on inflammation and intestinal microbiota disturbance induced by antibiotics in mice  

作　　者：Chun-lei Lu Byong H.Lee Yi-lin Ren Dan Ji Sheng-qi Rao Hua-xiang Li Zhen-quan Yang 

机构地区：[1]College of Food Science and Engineering,Yangzhou University,Yangzhou,225009,China [2]HEM Pharma Business Incubator Center 103,Handong Global University,Pohang,37554,Republic of Korea [3]Department of Gastroenterology,Affiliated Hospital of Jiangnan University,Wuxi,214041,China [4]Jiangsu Key Laboratory of Dairy Biotechnology and Safety Control,Yangzhou University,Yangzhou,225009,China

出　　处：《Food Bioscience》2022年第6期1645-1652,共8页食品生物科学（英文）

基　　金：financially supported by the National Natural Science Foundation of China(Grant numbers:32001661 and 32101964);the Natural Science Foundation of Jiangsu Province,China(Grant number:BK20190890).

摘　　要：Antrodia cinnamomea is an important edible and medicinal mushroom,and it exhibits multiple biological activities,such as hepatoprotection,antitumor,antivirus,and immunoregulation.Polysaccharides are the main products of A.cinnamomea in submerged fermentation.In this work,exopolysaccharides from A.cinnamomea(AEPS)were extracted and purified by alcohol precipitation and Sevag method.Composition analysis revealed that AEPS were primarily composed of three distinct polysaccharides with mean molecular weights of 1,013,233,and 28,743 kDa,accounting for 78%,18%,and 4%of AEPS,respectively.The AEPS haveβ-type glycosidic bonds and relatively strong resistance to digestion.In vivo experiments showed that the intragastric administration of AEPS in mice with medium dose(0.25 g/kg body weight of mice)has the following effects:remarkably alleviate lincomycin hydrochloride(LIH)-induced injuries to immune organs;enhance the relative abundance of beneficial microorganisms such as Lactobacillus,Roseburia,Ligilactobacillus,and Lachnospiraceae_NK4A136_group in the intestinal tract;greatly reduce the levels of inflammatory cytokines IL-6 and TNF-αin serum and the relative abundances of harmful microbes such as Enterococcus and Shigella;regulate the balance of the gut microflora;and relieve LIH-induced symptoms such as diarrhea,inflammation,and weight loss.These findings might represent a new alternative to develop novel multifunctional carbohydrate prebiotics.

关 键 词：Antrodia cinnamomea POLYSACCHARIDES Antibiotic-associated diarrhea INFLAMMATION Intestinal flora 

分 类 号：R574[医药卫生—消化系统]