Red algae-derived isofloridoside activates the sweet taste receptor T1R2/T1R3  

作　　者：Miku Akishino Yuko Aoki Hajime Baba Makio Asakawa Yoichiro Hama Susumu Mitsutake 

机构地区：[1]Graduate School of Advanced Health Sciences,Saga University,Saga,Japan [2]Graduate School of Agriculture,Saga University,Saga,Japan [3]Department of Applied Biochemistry and Food Science,Faculty of Agriculture,Saga University,Saga,Japan [4]Department of Food Science,Faculty of Education,Kumamoto University,Kumamoto,Japan [5]The United Graduate School of Agricultural Sciences,Kagoshima University,Kagoshima,Japan

出　　处：《Food Bioscience》2022年第6期1876-1881,共6页食品生物科学（英文）

基　　金：supported in part by a Grant-in-Aid for Scientific Reaserch(C)20K05927 from JSPS KAKENHI,Japan.

摘　　要：Isofloridoside (IF) is a naturally occurring galactosylglycerol compound with a refreshing sweet taste. It is found in red algae, where it acts as a photosynthetic intermediate. However, the mechanism by which IF elicits sweetness has not been determined. In this study, we investigate the interaction between IF and the sweet taste receptor T1R2/T1R3. We show that IF causes an increase in intracellular Ca2+ concentration and an increase in Erk phosphorylation in T1R2/T1R3-expressing HEK293T cells, indicating that IF interacts with T1R2/T1R3. IF also activates endogenously expressed T1R2/T1R3 in mouse small intestinal endocrine L-cells, and promotes the secretion of the incretin glucagon-like peptide-1 (GLP-1). In silico docking simulations of IF and T1R2/T1R3 predict that IF forms hydrogen bonds with Tyr103 in the extracellular Venus flytrap domain of T1R2. This result is consistent with the binding mode of many other sweet-tasting molecules. We found that IF have the potential as a new alternative sweetener.

关 键 词：GPCR Taste receptor T1R2 T1R3 FLORIDOSIDE Pyropia yezoensis 

分 类 号：TS201.2[轻工技术与工程—食品科学]