产蛋白酶海洋芽孢杆菌的分离鉴定及鲬鱼抗氧化肽的制备  

Isolation and Identification of Protease-Producing Marine Strain Bacillus sp.and Preparation of Antioxidant Peptides from Platycephalus indicus

作　　者：胡蓉 程钰 李强 蒋蕾 何跃辉 卢静 王淑军 HU Rong;CHENG Yu;LI Qiang;JIANG Lei;HE Yuehui;LU Jing;WANG Shujun(Key Laboratory of Marine Biological Resources and Environment of Jiangsu Province,Jiangsu Ocean University,Lianyungang 222005,Jiangsu,China;Key Laboratory of Marine Biotechnology of Jiangsu Province,Jiangsu Ocean University,Lianyungang 222005,Jiangsu,China;School of Life Science,Shanxi University,Taiyuan 237016,Shanxi,China)

机构地区：[1]江苏海洋大学江苏省海洋生物资源与环境重点实验室,江苏连云港222005 [2]江苏海洋大学江苏省海洋生物技术重点实验室,江苏连云港222005 [3]山西大学生命科学学院,山西太原237016

出　　处：《食品研究与开发》2024年第23期178-185,共8页Food Research and Development

基　　金：国家自然科学基金青年基金项目(32201964);江苏省高等学校基础科学(自然科学)面上项目(21KJB550003);2021年度连云港市第六期“521工程”科研项目(LYG06521202107)。

摘　　要：蛋白酶水解鱼类加工副产物,制备抗氧化活性肽,是提高其附加值的重要途径。该文从海州湾海泥中筛选得到一株产蛋白酶菌株H-1,结合形态鉴定、生理生化反应和16S rDNA基因序列分析鉴定为芽孢杆菌(Bacillus siamensis)。菌株H-1产蛋白酶的最适反应温度为55℃、pH值为8,具有较好的温度、pH稳定性,在谷胱甘肽、β-巯基乙醇等还原剂中具有良好的稳定性。50 mmol/L的金属离子Co^(2+)、Mn^(2+)、K^(+)、Mg^(2+)对酶活力有明显促进作用,Fe^(3+)、Ba^(2+)、Zn^(2+)对酶活力有抑制作用。该酶水解鲬鱼加工副产物制备具有抗氧化作用的活性肽,在浓度为10 mg/mL时,DPPH自由基清除率为80%、羟基自由基清除率为80%、超氧阴离子自由基清除率为52.7%,还原力为0.51。结果表明,该酶可以明显提高鲬鱼副产物的应用价值。Preparing antioxidant peptides by digestion with protease is an important way to increase the value added of fish by-products.A protease-producing strain H-1 was isolated from the sea mud of Haizhou Bay and identified as Bacillus siamensis based on the morphological,physiological,biochemical,and 16S rDNA gene sequence evidence.The optimal reaction conditions of the protease produced by strain H-1 were 55 ℃ and pH8.The protease had good thermostability and pH stability and demonstrated good stability in solvents such as glutathione,β-mercaptoethanol.The protease activity was improved by 50 mmol/L Co~(2+),Mn~(2+),K+,and Mg~(2+),while it was inhibited by Fe3+,Ba~(2+),and Zn~(2+).This enzyme hydrolyzed the by-products of Platycephalus indicus to produce active peptides with antioxidant properties.At a concentration of 10 mg/mL,the antioxidant peptides showed the DPPH,hydroxyl,and superoxide anion free radical clearance rates and the reducing power of 80%,80%,52.7%,and 0.51,respectively.The results indicated that the protease significantly enhanced the application value of the by-products of P.indicus.

关 键 词：产蛋白酶 芽孢杆菌 鲬鱼加工副产物 酶解 抗氧化 

分 类 号：TS201.3[轻工技术与工程—食品科学] TS254.9[轻工技术与工程—食品科学与工程]