产角蛋白酶芽孢杆菌50-3的分离与初步鉴定  

Isolation and Identification of Bacillus sp.50-3 with a High Yield of Keratinase

作　　者：岳晓禹[1] 常惟丹 崔丽伟[1] 刘安琪 李长滨[1] 许文涛[2] YUE Xiaoyu;CHANG Weidan;CUI Liwei;LIU Anqi;LI Changbin;XU Wentao(College of Food and Biotechnology Engineering,Henan University of Animal Husbandry and Economy,Zhengzhou 450046,Henan,China;Department of Nutrition and Health,China Agricultural University,Beijing 100091,China)

机构地区：[1]河南牧业经济学院食品与生物工程学院,河南郑州450046 [2]中国农业大学营养与健康系,北京100091

出　　处：《食品研究与开发》2024年第23期203-208,共6页Food Research and Development

基　　金：河南省科技发展计划项目(232102111068);河南省高等学校重点科研项目(22A550012)。

摘　　要：为获得产角蛋白酶活性较高的菌株,以1 942株动物粪便源芽孢杆菌为对象,在角蛋白酶筛选培养基中进行初筛,以菌株生长的透明圈与菌落直径比值较大的菌株在种子培养基中进行复筛,并进行菌株鉴定。结果表明:复筛得到的菌株50-3在鸡毛粉作为角蛋白酶诱导底物的培养基上,37℃发酵36 h,角蛋白酶活力可以达到(694±15)U/mL。通过形态观察、生理生化分析、全细胞蛋白电泳和16S rDNA序列分析,初步鉴定菌株50-3为枯草芽孢杆菌进化分支中的一个种,命名为Bacillus sp.50-3。A total of 1 942 strains of Bacillus from animal feces were cultured for the preliminary screening of the strains with high keratinase activity.The strains with large ratios of hydrolysis zone diameter to colony diameter were re-screened in seed media and identified.The results showed that strain 50-3 fermented at 37 ℃for 36 h in the medium with only chicken hair powder for inducing keratinase production reached the keratinase activity of(694±15)U/mL.According to morphological characteristics,physiological and biochemical properties,whole-cell protein electrophoresis,and 16S rDNA sequencing,strain 50-3 was preliminarily identified as a species in the clade of Bacillus subtilis in the phylogenetic tree and named Bacillus sp.50-3.

关 键 词：角蛋白酶 芽孢杆菌 分离 鉴定 角蛋白 

分 类 号：TQ925.2[轻工技术与工程—发酵工程]