酶解法提取夏枯草中多糖的工艺研究  

Study on the Extraction Process of Polysaccharide from Prunellae Spica by Enzymatic Hydrolysis

作　　者：赵玉荣 许金玉 侯宪邦 陆姗姗 ZHAO Yurong;XU Jinyu;HOU Xianbang;LU Shanshan(Hanlin College,Nanjing University of Chinese Medicine,Taizhou Jiangsu 225300,China)

机构地区：[1]南京中医药大学翰林学院,江苏泰州225300

出　　处：《药品评价》2024年第8期946-950,共5页Drug Evaluation

基　　金：泰州市“凤城英才”人才项目(泰科协发[2022]64号)。

摘　　要：目的探究酶解法提取夏枯草中水溶性多糖的工艺。方法以多糖提取率为指标,通过单因素试验探究酶的种类、提取温度、提取pH、酶添加量、反应时间的影响,并结合响应面法对提取条件优化,并分别对水解和酶解得到的多糖进行抗氧化活性比较。结果最佳提取条件为:酶解温度52℃,pH值6.1,纤维素酶加入量0.22 g,酶解时间107 min,在此条件下的夏枯草多糖提取率为(7.59±1.36)%。且试验发现,水解多糖和酶解多糖对DPPH显示出差异化的清除能力,其半数抑制浓度(IC_(50))分别为0.538 mg/mL和0.062 mg/mL。结论本研究提高了夏枯草多糖的提取率,为其临床应用提供了良好的理论依据。Objective The purpose of this study was to explore the process of enzymatic extraction of water-soluble polysaccharides from prunellae spica.Methods With the extraction rate of polysaccharide as the index,the effects of enzyme type,extraction temperature,extraction pH,enzyme addition amount and reaction time were investigated by single factor experiment.The extraction conditions were optimized by response surface method,and the antioxidant activity of polysaccharides obtained enzymolysis and hydrolysis was compared.Results The optimum extraction conditions were as follows:enzymolysis temperature 52℃,pH 6.1,cellulase dosage 0.22 g,enzymolysis time 107 min.Under these conditions,the extraction rate of prunella polysaccharide was(7.59±1.36)%.The results showed that the DPPH scavenging ability of prunella polysaccharides enzymolysis and hydrolysis was different,and the half inhibitory concentration(IC_(50))was 0.538 mg/mL and 0.062 mg/mL,respectively.Conclusion This study improved the extraction rate of prunella polysaccharide and provided a good theoretical basis for its clinical application.

关 键 词：夏枯草 酶解提取 多糖含量 抗氧化活性 

分 类 号：R284.2[医药卫生—中药学]