Evaluation indicators of Traditional Chinese Medicine syndromes for gouty arthritis with damp heat accumulation and the effect of administering Tongfeng Qingxiao formula(痛风清消方)  

作　　者：ZHANG Xiaoyun LI Yongjin LI Huanan CHAI Yuan CHENG Feng LI Binglin ZHOU Yi LAI Yu 

机构地区：[1]College for Clinical Medicine,Jiangxi University of Chinese Medicine,Nanning 53001l1,China [2]Department of Orthopedics,Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine,Nanchang 330006,China [3]Graduate school,Guangxi University of Chinese Medicine,Nanning 53001l,China [4]Department of Orthopedics,Affiliated Hospital of Jiangxi University of Chinese Medicine,Nanning 530000,China

出　　处：《Journal of Traditional Chinese Medicine》2024年第6期1204-1216,共13页中医杂志（英文版）

基　　金：the National Natural Science Foundation of China:a Study on the Use of Tongfengqingxiao for Treating Gouty Arthritis and Neutrophil Extracellular Traps Formation based on the Transforming Growth Factor-β1 Signaling Pathway (No. 81860857);a Study on the Mechanism by which Tongfeng Qingxiao Regulates the Toll-like receptors-myeloid Differentiation Factor 88-nuclear Factor Kappa-B Signaling Pathway in Gouty Arthritis Rats with Gut Dysbiosis and Dampness-Heat Syndrome based on the Gut-enteric-microbiota Axis (No. 82060871);National Natural Science Foundation of Jiangxi Province:a Study on the Regulatory Mechanism of the Toll-like receptors-Myeloid differentiation factor 88-nuclear factor kappa-B Signaling Pathway Related to Dysbacteriosis of the Intestinal Flora with Gouty Arthritis in Rats Mediated by Tongfengqingxiao,based on the “Gut-Microbiota” Axis (No. 20202BAB206071)。

摘　　要：OBJECTIVE: To evaluate the indicators of an animal model of gouty arthritis(GA) with dampness heat accumulation and the intervention effect of Tongfeng Qingxiao formula(痛风清消方, TFQXF). METHODS: Seventy-two healthy adult Sprague-Dawley male rats were selected and randomly divided into a normal group, model group, low-dose group, mediumdose group, high-dose group, and diclofenac group using a random number table method, with 12 rats in each group. After group intervention, the general condition of the rats in each group was monitored and recorded, and the swelling index was measured. After separating the serum, the changes in glutamic pyruvic transaminase (ALT), glutamic oxaloacetic transaminase(AST), carbamide(UREA), creatinine(CREA), triglyceride(TG), total serum cholesterol(TC), low density lipoprotein cholesterol(LDL-C), and high density lipoprotein cholesterol(HDL-C) in the serum of the rats in each group were measured using an automatic biochemical analyzer. The levels of motilin(MTL), gastrin(GAS), endothelin(ET), calcitonin gene-related peptide(CGRP), heat shock protein 70(HSP70), interleukin(IL)-1β, and nuclear factor kappa-B(NF-κB) in the serum of the rats in each group were evaluated using enzyme-linked immunosorbent assay(ELISA) kits. Kidney tissues were used to evaluate the protein and m RNA expression of aquaporin(AQP) 1 and AQP2. Colon tissue was used to evaluate the protein and m RNA expression of AQP3 and AQP4 by Western blotting(WB) assay and real-time quantitative polymerase chain reaction(RT-q PCR). The levels of ALT, AST, UREA, and CREA were used to evaluate the liver and kidney function of rats. The levels of MTL and GAS were used to evaluate the gastrointestinal function of rats. The levels of TG, TC, LDL-C, HDL-C, AQP1, AQP2, AQP3, and AQP4 were used to evaluate the "dampness" syndrome performance in rats. The levels of ET, CGRP, and HSP70 were used to evaluate the "heat" syndrome performance in rats. The levels of IL-1β and NF-κB were used to evaluate the degree of inflammation

关 键 词：ARTHRITIS GOUTY uric acid damp heat accumulation Tongfeng Qingxiao formula 

分 类 号：R259[医药卫生—中西医结合]