2005-2019年江苏省小肠结肠耶尔森菌的病原学和遗传学特征研究  

Pathogenic and genetic characteristics of Yersinia enterocolitica in Jiangsu Province from 2005 to 2019

作　　者：傅明慧[1] 王艳 朱小娟[1] 周璐[1] 刘静娴[1] 徐娅雯 杨华富[1] 崔仑标[1] 徐勤 吴斌[1] FU Ming-hui;WANG Yan;ZHU Xiao-juan;ZHOU Lu;LIU Jing-xian;XU Ya-wen;YANG Hua-fu;CUI Lun-biao;XU Qin;WU Bin(Jiangsu Center for Disease Control and Prevention,Nanjing 210009,China;Yangzhou Center for Disease Control and Prevention,Yangzhou 225000,China)

机构地区：[1]江苏省疾病预防控制中心(江苏省预防医学科学院),南京210009 [2]扬州市疾病预防控制中心,扬州225000

出　　处：《中国人兽共患病学报》2024年第11期1024-1030,1041,共8页Chinese Journal of Zoonoses

基　　金：国家自然科学青年基金项目(No.31700035);江苏省社会发展项目(No.BE2020719)。

摘　　要：目的了解2005-2019年江苏省分离的小肠结肠耶尔森菌的病原学和遗传学特征。方法对江苏省食源性疾病腹泻病人以及猪、狗、牛、羊、家禽、苍蝇和食品中分离得到的110株小肠结肠炎耶尔森菌进行毒力基因、生物血清型和耐药性分析,并在全基因组测序的基础上进行多位点序列分型(MLST)和核心基因组多位点序列分型(cgMLST)分析。结果110株小肠结肠炎耶尔森菌包括致病性菌株27株(24.5%),非致病性菌株83株(75.5%)。各种来源的菌株中,非致病性菌株占比均较高,其中93.8%的病人来源菌株(15/16)为非致病性菌株。致病性菌株的生物血清型主要为3/O∶3型(26/27,96.3%);非致病性菌株鉴定出了6种不同的生物血清型(不包含未知血清型),主要为1A/O∶8型(23/83,27.7%)和1A/O∶5型(14/83,16.9%)。110株菌对19种抗生素的敏感率达到80%以上。全基因组分析结果显示,致病性菌株均为ST135型,而非致病性菌株型别较为多样和分散。HierCC聚类分析将所有菌株聚类为3簇,致病性菌株单独成簇,病人来源菌株在各簇中均有分布。结论腹泻病人来源的小肠结肠耶尔森菌以非致病性菌株为主。相较于致病性菌株,非致病性菌株表现出了更为丰富的表观多样性和遗传多样性,应加强此类菌株的监测,警惕其感染人的风险。This study was aimed at understanding the etiological and genetic characteristics of Yersinia enterocolica isolated in Jiangsu Province between 2005 and 2019.All 110 identified strains of Y.enterocolica were from patients with foodborne diarrhea in Jiangsu Province,or from pigs,dogs,cattle,sheep,poultry,flies,or food.Virulence genes,biological serotypes,drug resistance,multilocus sequence typing(MLST),and core genome multilocus sequence typing(cgMLST)based on whole-genome sequencing were performed.The strains included 27 pathogenic strains(24.5%)and 83 non-pathogenic strains(75.5%).Non-pathogenic strains accounted for a high proportion,particularly among strains from patients(15/16,93.8%).The biological serotypes of pathogenic strains were mainly type 3/O∶3(26/27,96.3%).Non-pathogenic strains included 1A/O∶8 type(23/83,27.7%),1A/O∶5 type(14/83,16.9%),and the other four biological serotypes(excluding unknown serotypes).Pathogenic strains were dominated by type 3/O∶3(26/27,96.3%),and more than 80%of these strains were sensitive to 19 antibiotic types.Whole-genome sequencing indicated that the pathogenic strains were all ST135 type,whereas the non-pathogenic strains were more diverse and scattered.HierCC clustering analysis grouped all strains into three clusters:pathogenic strains were in one cluster,and strains from patients were found in all three clusters.In conclusion,the Y.enterocolica strains from patients were primarily non-pathogenic.Non-pathogenic strains showed richer epigenetic and genetic diversity than pathogenic strains.The monitoring of these strains should be strengthened to decrease the risk of human infection.

关 键 词：小肠结肠炎耶尔森菌 生物血清型 毒力基因 耐药性 全基因组测序 多位点序列分型 

分 类 号：R378[医药卫生—病原生物学]