Gastrodin ameliorates exercise-induced fatigue via modulating Nrf2 pathway and inhibiting inflammation in mice  

作　　者：Yaping Zhou Qi Wu Wen Yu Fan Ye Yunyun Cao Otobong DAkan Xiuxiu Wu Tiantian Xie Han Lu Fuliang Cao Feijun Luo Qinlu Lin 

机构地区：[1]National Engineering Center for Deep Process of Rice and Byproducts,Hunan Provincial Key Laboratory of Grain-oil Deep Process and Quality Control,Hunan Key Provincial Laboratory of Processed Food for Special Medical Purpose,College of Food Science and Engineering,Central South University of Forestry and Technology,No.498,Shaoshan Road,Changsha,410004,Hunan,PR China [2]Co-innovation Center for the Sustainable Forestry in Southern China,College of Forestry,Nanjing Forestry University,No.159,Longpan Road,Nanjing,210037,Jiangsu,PR China

出　　处：《Food Bioscience》2023年第1期745-761,共17页食品生物科学（英文）

基　　金：supported by Program for Science&Technology Innovation Platform of Hunan Province(2019TP102);Graduate Innovative Research Project of Hunan province and Central South University of Forestry and Technology(CX20210862,CX202101027);Science and Technology Plan Project of Changsha City(kq2014275).

摘　　要：Gastrodia elata Bl.is traditional Chinese medicine used to alleviate fatigue,but its underlying mechanism is still unclear.This study explored the anti-fatigue mechanism of gastrodin by exercise-induced fatigue model and network pharmacology.This study found that gastrodin(200 mg/kg/day)had significant anti-fatigue effects in C57BL/6J mice based on mouse energy and endurance measurements.Gastrodin could effectively ameliorate biochemical indexes in the fatigue mice.The putative targets of“Gastrodin”and“Fatigue”were obtained by integrating multiple databases,and a virtual network containing 220 interactive targets was constructed by STRING and Cytoscape.Functional annotation analysis of these targets by g:Profiler showed that they mainly contribute to the cellular processes,protein binding,and other functions and participate in metabolic pathways,cancer pathways,PI3K-Akt signaling pathway,etc.We found that oxidation and inflammatory factors played an important role in the virtual network of gastrodin anti-fatigue,which was supported by microarray dataset analysis and a molecular docking prediction.Additionally,real time-quantitative PCR and Western blot analysis indicated that gastrodin could promote the activation of the Nrf2 signal pathway,which could activate HO-1 and NQO1;gastrodin also could down-regulate the expressions of IL-1β,TNF-α,and IL-6.In summary,gastrodin can ameliorate exercise-induced fatigue by modulating the Nrf2 pathway and inhibiting expressions of inflammatory factors,this provides a new clue for the development of gastrodin-functional foods or anti-fatigue drugs.

关 键 词：ANTI-FATIGUE GASTRODIN Network pharmacology Molecular mechanism Nrf2 signaling pathway 

分 类 号：TS201[轻工技术与工程—食品科学]