A multifunctional key to open a new window on the path to natural resources-lessons from a study on chemical composition and biological capability of Paeonia mascula L.from Turkey  

作　　者：Inci Kurt-Celep Gokhan Zengin Engin Celep Stefano Dall’Acqua Stefania Sut Irene Ferrase Gunes Ak Abdullahi Ibrahim Uba Rıdvan Polat Deniz Canlı Ekrem Darendelioglu Muhammad Zakariyyah Aumeeruddy Mohammad Ali Shariati Mohamad Fawzi Mahomoodally 

机构地区：[1]Department of Pharmacognosy,Faculty of Pharmacy,Yeditepe University,34755,Istanbul,Turkey [2]Department of Biology,Science Faculty,Selcuk University,Konya,42300,Turkey [3]Department of Pharmacognosy,Faculty of Pharmacy,Acıbadem Mehmet Ali Aydınlar University,34752,Istanbul,Turkey [4]Department of Pharmaceutical and Pharmacological Sciences,University of Padova,Via Marzolo 5,35131,Padova,Italy [5]Department of Molecular Biology and Genetics,Faculty of Engineering and Natural Sciences,Kadir Has University,Istanbul,34083,Turkey [6]Department of Landscape Architecture,Faculty of Agriculture,Bingol University,12000,Bingol,Turkey [7]The Unit of Model University Coordination Center,Bingol Unviersity,12000,Bing¨ol,Turkey [8]Department of Molecular Biology and Genetics,Faculty of Art and Sciences,Bing¨ol University,Bingol,Turkey [9]Department of Health Sciences,Faculty of Medicine and Health Sciences,University of Mauritius,Reduit,80837,Mauritius [10]Department of Scientific Research,K.G.Razumovsky Moscow State University of Technologies and Management(The First Cossack University),73 Zemlyanoy Val,Moscow,109004,Russian Federation [11]Department of Scientific Research,Russian State Agrarian University-Moscow Timiryazev Agricultural Academy,49 Timiryazevskaya str.,Moscow,127550,Russian Federation [12]Center for Transdisciplinary Research,Department of Pharmacology,Saveetha Dental College,Saveetha,Institute of Medical and Technical Science,Chennai,600077,India [13]Centre of Excellence for Pharmaceutical Sciences(Pharmacen),North-West University,Potchefstroom,2520,South Africa

出　　处：《Food Bioscience》2023年第1期1301-1316,共16页食品生物科学（英文）

摘　　要：The genus Paeonia has gained great interest from the scientific community for exploration as a source of bioactive compounds.The current work focused on the chemical characterisation and biological properties of Paeonia mascula L.from Turkey.The chemical characterisation of the extracts was analysed by LC-DAD-ESI and LC-APCI-MS.The antioxidant,enzyme inhibitory and anticancer properties were evaluated in vitro.The methanol extract was the most effective as antioxidant,metal chelator and was the most effective inhibitor of acetylcholinesterase(AChE)and tyrosinase.The chloroform extract showed highest inhibition of butrylcholinesterase(BChE)and amylase.Ethyl acetate extract displayed glucosidase inhibition.The most abundant compounds were hexagalloyl glucose,penta galloyl glucose,gallic acid,tetragalloyl glucose,paeoniflorin and the most abundant flavonoid was quercetin-3-O-glucoside.β-sitosterol was the most abundant phytosterol.The anticancer effect of P.mascula was evaluated by using HELA cells evaluating different pathways.The possible inhibition effect of P.mascula on the reactive oxygen species(ROS)pathway was controlled by performing the advanced glycation end product(AGE).To determine the effect of P.mascula on active molecular pathways in the HELA cancer cell line,the levels of Phospho-NF-Kβp65,AGE receptor RAGE protein,antiapoptotic BCL-2,proapoptotic BAX and P-53 proteins were checked,respectively.Phospho-NF-Kβp65,RAGE and BCL-2,which are both ROS inducers and play an active role in cancer prognosis,were found to have decreased levels after the application of P.mascula.Our findings provide a scientific basis for Paeonia mascula,which may serve as a source of naturally occurring bioactive compounds for healthpromoting applications.

关 键 词：PAEONIA Phenolics Antioxidant Enzyme inhibition ANTICANCER Insilico analysis 

分 类 号：TS201.2[轻工技术与工程—食品科学]