Bioengineered silver nanoparticles induced apoptosis through upregulation of caspase 3 and caspase 8 proteins in breast adenocarcinoma MDA-MB-231 cells and impede angiogenesis  

作　　者：Shahnaz Majeed Nurul Izzah Binti Abu Bakar Mohammad Danish Afzan Binti Mahmad Mohamad Nasir Mohamad Ibrahim Norul Aini Zakariya Sreenivas Patro Sisinthy Ravindran Muthukumarasamy Abdulaziz M.Alanazi Mohammed Tahir Ansari Ohoud A.Jefri 

机构地区：[1]Faculty of Pharmacy and Health Sciences,Universiti Kuala Lumpur,Royal College of Medicine Perak,Ipoh,30450,Malaysia [2]Bioresource Technology Section,School of Industrial Technology,Universiti Sains Malaysia,11800,Penang,Malaysia [3]Laboratory Department,Universiti Kuala Lumpur,Royal College of Medicine Perak,Ipoh,30450,Malaysia [4]School of Chemical Sciences,Universiti Sains Malaysia,11800,Penang,Malaysia [5]School of Pharmacy,Faculty of Science and Engineering,University of Nottingham Malaysia,Semenyih-43500,Selangor,Malaysia [6]Department of Chemistry,Faculty of Science,Islamic University of Madinah,Madinah,42351,Saudi Arabia [7]Department of Biological Science,Faculty of Science,King Abdulaziz University Jeddah,21589,Saudi Arabia [8]Department of Biology,Faculty of Science,Taibah University,Al-Madinah Al Munawarah,Saudi Arabia [9]Nanotechnology Group,Faculty of Science and Engineering,University of Nottingham Malaysia,Semenyih-43500,Selangor,Malaysia

出　　处：《Particuology》2024年第12期252-264,共13页颗粒学报（英文版）

摘　　要：In recent years,a lot of research has been done on silver nanoparticles(SNP)due to their numerous applications in the biomedical,pharmaceutical,and drug delivery industries.In this present study SNP were green synthesized using Melicope lunu-ankenda(M.lunu-ankenda)leaf extract.The addition of AgNO3 causes a color change.L-arginine addition results in further colour changes confirming conjugation.A UV–Vis spectrophotometric examination showed that the absorption peak for SNP was 435 nm,while the peak for L-arginine SNP(cSNP)was 422 nm.FTIR analysis confirmed the association of amides and amines with nanoparticles.The spherical nature of the silver was disclosed by SEM,and its elemental character is verified by EDS.The thermal stability of the nanoparticles is determined by TGA analysis,while TEM examination verifies their spherical shape.Using the MTT assay,these cSNP exhibited outstanding toxicity analysis(IC5038.72μg/ml)against MDA-MB-231 cells.These cSNP causes damage to the mitochondria(JC1 staining),which causes oxidative stress and the production of ROS with 83%of DCF expression in cancer cells.Furthermore,as demonstrated by the Comet assay and DAPI,these cSNP cause good DNA damage in the treated cells.Additionally,using flow cytometry,cSNPs potentially trigger apoptosis by triggering the expression of caspase 3 and caspase 8 proteins.Additionally,through CAM,cSNP demonstrated strong anti-angiogenesis activity by reducing the number of blood vessel branches.These findings suggest that cSNP may be crucial for drug delivery and cancer treatment.

关 键 词：Silver nanoparticles TEM ANTI-ANGIOGENESIS DAPI ROS 

分 类 号：R737.9[医药卫生—肿瘤]