Controllable Histotomy Based on Hierarchical Magnetic Microneedle Array Robots  

作　　者：Xiaoxuan Zhang Hanxu Chen Taiyu Song Jinglin Wang Yuanjin Zhao 

机构地区：[1]Department of Rheumatology and Immunology,Nanjing Drum Tower Hospital,School of Biological Science and Medical Engineering,Southeast University,Nanjing 210096,China [2]Oujiang Laboratory(Zhejiang Lab for Regenerative Medicine,Vision and Brain Health),Wenzhou Institute,University of Chinese Academy of Sciences,Wenzhou 325001,China

出　　处：《Engineering》2024年第11期166-174,共9页工程（英文）

基　　金：supported by the National Key Research and Development Program of China(2020YFA0908200);the National Natural Science Foundation of China(T2225003,52073060,61927805);the Nanjing Medical Science and Technique Development Foundation(ZKX21019);the Clinical Trials from Nanjing Drum Tower Hospital(2022-LCYJ-ZD-01);the Guangdong Basic and Applied Basic Research Foundation(2021B1515120054).

摘　　要：Investigation of patient-derived primary tissues is of great importance in the biomedical field,but recent tissue slicing and cultivation techniques still have difficulties in satisfying clinical requirements.Here,we propose a controllable histotomy strategy that utilizes hierarchical magnetic microneedle array robots to tailor primary tissues and establish the desired high-throughput tissue-on-a-chip.This histotomy is performed using a three-dimensional printed,mortise-tenon-structured slicing device coupled with a magnetic-particle-loaded and pagoda-shaped microneedle array scaffold.Due to the multilayered struc-ture of these microneedles,tissue specimens can be fixed onto the microneedle scaffold via mechanical interlocking,thereby effectively avoiding tissue slipping during the slicing process.Owing to the encapsu-lation of magnetic microneedle fragments,these tissue pieces can act as magnetically responsive biohybrid microrobots and can be easily manipulated by magnetic fields,facilitating their separation,transportation,and dynamic culture.Using this strategy,we demonstrate that primary pancreatic cancer tissues can be tailored into tiny pieces and cultured in multilayered microfluidic chips for efficient high-throughput drug screening,indicating the promising future of this technique’s application in clinical settings.

关 键 词：Histotomy Drug screening MICRONEEDLE MICROROBOT Tumor-on-a-chip High throughput 

分 类 号：R318[医药卫生—生物医学工程] TP242[医药卫生—基础医学]