艾叶精油微囊的制备及其生物活性检测  

Preparation and biological activity detection of Artemisia argyi essential oil microcapsules

作　　者：王海燕[1,2] 罗伟 秦淑娟 郭磊磊 叶兆伟 陈琼[1,2] WANG Hai-yan;LUO Wei;QIN Shu-juan;GUO Lei-lei;YE Zhao-wei;CHEN Qiong

机构地区：[1]信阳农林学院药学院,河南信阳464000 [2]河南省大别山林药生态种植工程技术研究中心,河南信阳464000 [3]新乡海滨药业有限公司,河南新乡453000

出　　处：《饲料研究》2024年第21期104-108,共5页Feed Research

基　　金：河南省重点研发专项(项目编号:No.241111311400);河南省科技攻关项目(项目编号:242102310575);信阳农林学院科技创新团队项目(项目编号:XNKJTD-008)。

摘　　要：试验以艾叶精油初乳为囊芯物、明胶为囊材、聚乙二醇4000为添加剂,在单因素试验的基础上,通过正交试验设计,对艾叶精油微囊的制备工艺进行优化,并检测其体外抗氧化活性和抑菌活性。结果显示,艾叶精油微囊的最佳制备工艺条件为芯材比1.0∶3.0,聚乙二醇4000用量1.5%,喷雾温度55℃、蠕动速度6 r/min,在此条件下包封率为(86.58±1.28)%,载药量为(15.54±1.56)%,综合评分达到95.21±1.08。此工艺制备的艾叶精油微囊具有一定的体外抗氧化活性,当微囊质量浓度为3.0 g/L时,对1,1-二苯基-2-三硝基苯肼(DPPH)和羟基自由基的清除率分别达到(75.56±2.01)%和(80.12±0.96)%。艾叶精油微囊对金黄色葡萄球菌和甲型副伤寒沙门氏菌具有较好的抑菌效果,且对金黄色葡萄球菌的抑菌效果优于对甲型副伤寒沙门氏菌的抑菌效果。研究表明,艾叶精油微囊具有较高的应用价值,可为其用作畜禽功能饲料添加剂的开发提供参考。Using Artemisia argyi essential oil colostrum as the core material,gelatin as the capsule material,and polyethylene glycol 4000 as the additive,the preparation process of Artemisia argyi essential oil microcapsules was optimized based on single factor experiments and orthogonal experimental design,and the in vitro antioxidant and antibacterial activities of Artemisia argyi essential oil microcapsules were tested.The results showed that the best preparation conditions of Artemisia argyi essential oil microcapsules were the ratio of core material to capsule material of 1.0∶3.0,the dosage of polyethylene glycol 4000 of 1.5%,spray temperature 55℃,peristaltic speed of 6 r/min.Under these conditions,the encapsulation rate was(86.58±1.28)%,the drug loading capacity was(15.54±1.56)%,and the comprehensive score was 95.21±1.08.The Artemisia argyi essential oil microcapsules prepared by this process have a certain antioxidant capacity.When the mass concentration of the microcapsules was 3.0 g/L,the clearance rates of DPPH and hydroxyl radical were(75.56±2.01)%and(80.12±0.96)%,respectively.Artemisia argyi essential oil microcapsules had a good antibacterial effect on Staphylococcus aureus and Salmonella paratyphi A,and its antibacterial effect on Staphylococcus aureus was better than that on Salmonella paratyphi A.The study indicates that Artemisia argyi essential oil microcapsules have high application value,which can provide reference for the development of functional feed additives for livestock and poultry.

关 键 词：艾叶精油 微囊 抗氧化活性 抑菌活性 

分 类 号：S816.7[农业科学—饲料科学]