Antifungal activity of diacetyl, a volatile organic compound, on Trichoderma lixii F2 isolated from postharvest Lanzhou lily bulbs  

作　　者：Lijun Ling Mingmei Pang Hong Luo Wenting Cheng KunLing Jiang Yuanyuan Wang 

机构地区：[1]College of Life Sciences,Northwest Normal University,Lanzhou,730070,PR China [2]The Rural Development Academy,Northwest Normal University,PR China

出　　处：《Food Bioscience》2023年第2期193-202,共10页食品生物科学（英文）

基　　金：This work was supported by the Lanzhou Science and Technology Plan Project[grant number:2018-1-104];the Special Fund Project for Guiding Science and Technology Innovation and Development in Gansu Province[grant number:2019ZX-05];the Gansu Province Colleges and Universities Industrial Support Plan Project[grant number:2020C-21].

摘　　要：The most severe fungal disease is postharvest gray mold,which might cause massive losses in Lanzhou lily bulbs.Some microorganism-produced volatile organic compounds(VOCs)have been proven to have antifungal action and can be employed as fruit and vegetable preservation.Our most recent research discovered that diacetyl(2,3-butanedione)produced by Bacillus subtilis CL2 could significantly prevent some pathogens.The goal of this investigation was to see if diacetyl had antifungal properties against Trichoderma lixii F2,one of the most common postharvest rot pathogens of Lanzhou lily bulbs.The results indicated that diacetyl(1.587μL/mL)fumigation treatment significantly inhibited the hyphal growth,modified hyphal morphology,and disrupted the cell membrane integrity.In vivo tests the antifungal mechanisms of diacetyl on T.lixii F2 were performed in Lanzhou lily bulbs.The results indicated that the content of total phenols and flavonoids increased,while malondialdehyde decreased under different treatment by diacetyl.Meanwhile,the activities of peroxidase,polyphenol oxidase,catalase,and superoxide dismutase and phenylalanine ammonia-lyase in Lanzhou lily bulbs were investigated.It was showed that diacetyl could eliminate excessive reactive oxygen species,reduce the damage of plant cells,and improve the resistance of Lanzhou lily bulbs against T.lixii F2.This research reveals that diacetyl could activate antioxidant enzymes and disease-resistant enzymes in Lanzhou lily bulbs to prevent pathogenic fungus from invading and inducing resistance,laying the groundwork for future uses in preserving Lanzhou lily bulbs.

关 键 词：DIACETYL Lanzhou lily bulbs Postharvest preservation Volatile organic compounds Inhibitory effects 

分 类 号：TS201.3[轻工技术与工程—食品科学]