Novel ACE inhibitory peptides derived from bighead carp (Aristichthys nobilis) hydrolysates: Screening, inhibition mechanisms and the bioconjugation effect with graphene oxide  

作　　者：Mengting Chen Lan Wang Changliang Zheng Aimin Ma Kai Hu Aoli Xiang Zhida Sun Bijun Xie Guangquan Xiong Liu Shi Sheng Chen Wenjin Wu 

机构地区：[1]Key Laboratory of Agricultural Products Cold Chain Logistics,Ministry of Agriculture and Rural Affairs,Institute of Agro-Products Processing and Nuclear Agricultural Technology,Hubei Academy of Agricultural Sciences,Wuhan,430064,Hubei,China [2]College of Food Science and Technology,Huazhong Agricultural University,Wuhan,430070,Hubei,China

出　　处：《Food Bioscience》2023年第2期488-496,共9页食品生物科学（英文）

基　　金：The research was funded by the National Key R&D Program of China(2019YFD0901805).

摘　　要：The ACE inhibitory peptides have been characterized from the enzymatic digestion product of bighead carp protein and bioconjugated with graphene oxide(GO)to enhance its activity.The results showed that aspartic acid and glutamic acid had the highest levels in ultrafiltration fractions(<5 kDa),where eight potential ACE inhibitory peptides were also identified(ADSNHKAF,KLWHHTF,LLRLHF,PPSEPTKL,VEKFPLF,YLRLHF,YYKLKPLL,YYKLKPML).Among the eight peptides,YLRLHF showed the best ACE inhibitory activity(IC50=121.90μM)and was a competitive inhibitor.Molecular docking experiments showed that YLRLHF formed four hydrogen bond interactions in the ACE protein pocket,coordination bonding with Zn^(2+),and π-πconjugation interactions to His421.GO elevated the ACE inhibitory activity of YLRLHF(at 0.1 mg/mL)from 43.36%to 51.72%.The structural characterization results obtained from FI-IR,XPS,SEM,and TEM demonstrated the successful combination of GO and YLRLHF.Additionally,biocouples of ACE inhibitory peptides from bighead carp proteins with GO might be potential candidates for future functional foods and antihypertensive drugs.

关 键 词：ACE inhibitory activity Bioactive peptides Molecular docking Graphene oxide BIOCONJUGATION 

分 类 号：TS254[轻工技术与工程—水产品加工及贮藏工程]