Effects of solid-state fermentation using R. oligosporus on the phytochemical composition of wild-simulated ginseng leaf and its biological properties  

作　　者：Juho Lim Hayeong Kim Sung Bae Park Kunal Pal Seung Wook Kim Doman Kim 

机构地区：[1]Graduate School of International Agricultural Technology,Seoul National University,Gangwon-do,25354,Republic of Korea [2]Institute of Food Industrialization,Institutes of Green Bioscience and Technology,Seoul National University,Gangwon-do,25354,Republic of Korea [3]Biseondong Wild Ginseng Company,Hongcheon-gun,Gangwon-do,25162,Republic of Korea [4]Department of Biotechnology and Medical Engineering,National Institute of Technology,Rourkela,769008,India [5]Ottogi Corporation,Anyang-si,Gyeonggi-do,14060,Republic of Korea [6]Fervere Campus Corporation,Gangwon-do,25354,Republic of Korea

出　　处：《Food Bioscience》2023年第2期532-540,共9页食品生物科学（英文）

基　　金：This work was supported by the National Research Foundation of Korea,Republic of Korea(grant number 2022R1I1A1A01069773,2022R1F1A1063434,and 2022R1A6A3A13064354);the Technology Innovation Program(grant number 20009663);the Ministry of Trade,Industry&Energy(MOTIE,Republic of Korea);the OTTOGI Corporation,Republic of Korea,through the Research and Publication Project.

摘　　要：Wild-simulated ginseng(WSG)grown in the mountains without specific facilities is widely used as functional foods in Asian countries due to its bioactive compounds,such as ginsenosides.However,long-time cultivation,lower survival rate,and small size of WSG limit its use in the food industry.In this study,WSG leaves,which can be harvested annually,were fermented using R.oligosporus for 0-10 days.The phytochemical compounds of fermented WSG leaves,including squalene,phenols,saponins,and most ginsenosides(except for Rb1),were increased.L-carnitine was newly synthesized in WSG leaves,with a content of 165.1μg/g measured after 10 days of fermentation.Moreover,the minor ginsenosides Rg2,Rg3,Rh1,F1,F2,and CK increased by 1.32-,1.58-,1.42-,1.91-,1.49-,and 4.43-fold compared to non-fermented WSG leaves.The highest antioxidant activities,as measured by FRAP and ORAC assays of WSG leaves,were obtained after 3 days of fermentation.Furthermore,the suppression of NO release in LPS-stimulated RAW264.7 cells and lipid accumulation in mouse 3T3-L1 cells of fermented WSG leaves was greater than in WSG leaves.From these results,fermented WSG leaves have potential applications as functional materials in the food industry.

关 键 词：Wild-simulated ginseng leaf L-CARNITINE Solid-state fermentation R.oligosporus GINSENOSIDES Anti-inflammation Anti-lipid accumulation 

分 类 号：TS201.2[轻工技术与工程—食品科学]