Formation mechanism and stability of low environment-sensitive ternary nanoparticles based on zein-pea protein-pectin for astaxanthin delivery  

作　　者：Tianyu Shi Chengsheng Jia Xinshuo Wang Shuqin Xia Xingwei Wang Chunli Fan Xiaoming Zhang Caleb John Swing 

机构地区：[1]State Key Laboratory of Food Science and Technology,Jiangnan University,1800 Lihu Road,Wuxi,Jiangsu,214122,People’s Republic of China [2]School of Food Science and Technology,Collaborative Innovation Center of Food Safety and Quality Control,Jiangnan University,1800 Lihu Road,Wuxi,Jiangsu,214122,People’s Republic of China [3]Department of Animal Sciences,Colorado State University,350 W.Pitkin St.,Fort Collins,CO,80523-1171,USA

出　　处：《Food Bioscience》2023年第2期549-557,共9页食品生物科学（英文）

基　　金：All authors acknowledge the National Natural Science Foundation of China(32272244);program of“Collaborative innovation center of food safety and quality control in Jiangsu Province”.

摘　　要：This study was devoted for preparing zein-pea protein-pectin ternary nanoparticles through an alcohol-free pH-shifting method for the delivery of astaxanthin(Ax).The complexation of zein and pea protein(PP)in the weight ratio range of 1:2.5 to 1.2:1 maintained the dispersity at pH 7.0 with the average particle size of 90.47-132.38 nm and the polydispersity index of 0.204-0.245.The circular dichroism,fluorescence,Fourier transform infrared spectroscopy and other analyses indicated that the zein interacted with PP to form a composite structure through the folding action of protein structure in the process of pH reduction driven by hydrophobic and electrostatic forces.The incorporation of pectin(pec)further enhanced the physicochemical stability of the nanoparticles.The optimal weight ratio of zein/PP/pec was 2:4:3,and the encapsulation efficiency of astaxanthin reached 94.22%.The X-ray diffraction results showed that the astaxanthin was amorphous after embedding.Compared with Ax/zein-PP,the Ax/zein-PP-pec showed better colloidal stability under the conditions of wide pH range(4.0-8.0),high temperature(90℃),long time ultraviolet light treatment,and 15-day storage.These results indicated that the construction of the zein-PP-pec nanoparticles could be used as a low environment-sensitive nanocarrier for the loading,protection,and delivery of hydrophobic functional substances such as astaxanthin.

关 键 词：ZEIN Pea protein PECTIN ASTAXANTHIN Interaction force Low environment-sensitive 

分 类 号：TS201.2[轻工技术与工程—食品科学]