Development of ic-ELISA and colloidal cold-based immunochromatographic assay for red 2G detection in fruit drinks, red wine, and yoghurts  

作　　者：Xiaoling Li Xinxin Xu Lingling Guo Shanshan Song Liqiang Liu Yingyue Zhu Hua Kuang Chuanlai Xu Liguang Xu 

机构地区：[1]State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi,Jiangsu,214122,PR China [2]International Joint Research Laboratory for Biointerface and Biodetection,And School of Food Science and Technology,Jiangnan University,Wuxi,Jiangsu,214122,PR China [3]School of Biotechnology and Food Engineering,Changshu Institute of Technology,Changshu,Jiangsu,215500,PR China

出　　处：《Food Bioscience》2023年第2期865-871,共7页食品生物科学（英文）

基　　金：This work was funded by the National Key R&D Program(2019YFC1604602);grants from Science and Technology Program of Jiangsu Market Supervision and Administration Bureau(KJ21125093);This work was also supported by the Fundamental Research Funds for the Central Universities(JUSRP622012).

摘　　要：Red 2G(R2G),a cheap industrial colorant,cannot be added to food.An anti-R2G monoclonal antibody(mAb)was prepared by immunizing mice with the conjugate of R2G hapten and protein,which based on the 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide(EDC)method.Indirect competitive enzyme-linked immunosorbent assay(ic-ELISA)and colloidal gold-based immunochromatographic assay(CG-ICA)methods were used to determine R2G in fruit drinks,red wine,and yoghurts.A standard curve of the developed ic-ELISA showed that the IC50 of the anti-R2G mAb was 1.02 ng/mL,and limit of detection value(LOD)was 0.21 ng/mL.For the CG-ICA developed,the visual limit of detection values(vLOD)were 2 ng/mL and cut-off values of 100 ng/mL in samples.The results indicated that these two methods could be used to quickly detect R2G in fruit drinks,red wine,and yoghurts.

关 键 词：Monoclonal antibody Red 2G Ic-ELISA HAPTEN Colloidal gold-based immunochromatographic assay 

分 类 号：TS201[轻工技术与工程—食品科学]