Phenolic compounds-containing fruit peel extracts of Garcinia humilis exhibit anti-melanoma activity  

作　　者：Mahesha M.Poojary Thi Dao Nguyen Chaitali Dekiwadia Daniel A.Dias Tien Huynh 

机构地区：[1]Department of Food Science,University of Copenhagen,Rolighedsvej 26,1958,Frederiksberg C,Denmark [2]Discipline of Laboratory Medicine,School of Health and Biomedical Sciences,RMIT University,PO Box 71,Bundoora,3083,Victoria,Australia [3]School of Biosciences and Food Technology,RMIT University,PO Box 71,Bundoora,3083,Victoria,Australia [4]RMIT Microscopy and Microanalysis Facility,GPO 2476,Melbourne,3001,Victoria,Australia [5]Centre for Advanced Sensory Science(CASS),School of Exercise and Nutrition Sciences,Deakin University,Melbourne Burwood Campus,221 Burwood Highway,Burwood,VIC 312,Australia

出　　处：《Food Bioscience》2023年第2期1433-1438,共6页食品生物科学（英文）

摘　　要：There has been considerable interest in commercializing fruits of Garcinia humilis due to its pleasant taste and nutritional quality.However,the industrial processing of the fruit generates the peel as waste and this study focussed on upcycling of the peel for its anti-melanoma activity at different extraction conditions(24℃ and 37℃ for 24 h,and 70℃ for 1 h).Although the extraction yield was highest at 70℃,the phenolics and flavonoids extracted,and anti-cancer activity was highest when extracted at 37℃.Polyphenolic compounds including epicatechin,procyanidins,mangostanol,and gamma-mangostin were tentatively identified based on LC-Orbitrap MS/MS analysis.These extracts showed dose-dependent anti-melanoma activity on MM418C1(BRAF mutated oncogene)and D24(wild type BRAF oncogene)cells with IC_(50) in the range of 130-200μg/mL and 122-176μg/mL.Furthermore,the extracts had little effect on control fibroblast cells,indicating selective activity on cancer cells,and displayed morphological changes indicative of apoptosis.Although these extracts were 10 fold less effective compared to anti-cancer drug controls,cisplatin and vemurafenib,the moderate anti-cancer activity of Garcinia peel provides future opportunities for upcycling of this discarded waste as a functional food ingredient.

关 键 词：Garcinia humilis Achacha Phenolics Anti-melanoma ANTICANCER Functional foods 

分 类 号：TS201.4[轻工技术与工程—食品科学]