基于响应面的生防真菌AH-1固体发酵条件优化  

Optical Conditions for Solid-state Fermentation of the Biocontrol Fungal AH-1 Based on Response Surface Methodology

作　　者：廖钫哲 白国栋 李如华[1] 严东辉[1] Liao Fangzhe;Bai Guodong;Li Ruhua;Yan Donghui(Key Laboratory of Forest Protection of National Forestry and Grassland Administration,Ecology and Nature Conserverion Institute,Chinese Academy of Forestry,Beijing 100091,China;Chifeng Forest and Grassland Protection Center,Inner Nangolia,Chifeng 024050,China)

机构地区：[1]中国林业科学研究院森林生态环境与自然保护研究所,国家林业和草原局森林保护学重点实验室,北京100091 [2]内蒙古赤峰市森林草原保护发展中心,赤峰024050

出　　处：《陆地生态系统与保护学报》2024年第4期27-33,77,共8页Terrestrial Ecosystem and Conservation

基　　金：“十四五”国家重点研发计划课题(2022YFD1401003)。

摘　　要：【目的】为开发新的森林病害的微生物生防制剂,探明和优化真菌生防菌株AH-1的固体发酵条件。【方法】使用单因素测试及响应面法优化发酵条件。【结果】结果表明,影响固体发酵的3个主要因素是接种量、麦麸含量和温度;优化后的固体发酵培养基组成为麦麸∶玉米粉约3∶2。优化后的固体发酵培养条件为:初始pH值7、含水量60%,粘帚菌种子液接种量约18%(孢子浓度为1×10^(6)个/mL),温度30℃,发酵天数10 d。此条件下的产孢量为1.672×10^(10)个孢子/g,较优化前的单因素试验产孢量提高约2~3倍。【结论】用响应曲面法优化的发酵条件,能明显提高固体发酵的生防菌株AH-1的产孢量,为其规模化生产和菌剂制备奠定了基础。【Objective】In order to develop new biocontrol agent,the conditions of solid-state fermentation of biocontrol strain AH-1 were explored and optimized.【Methods】Singal factor assessment and response surface methodology were performed.【Result】The results showed that the three main factors affecting solid-state fermentation were inoculation amount,wheat bran content and temperature.The optimized solid-state fermentation medium composition was wheat bran:corn flour of 3:2.The optimized solid-state fermentation culture conditions were as follows:initial pH-value of 7,water content of 60%,spore suspension inoculation amount of about 18%(with concentration of 1×10^(6)conidia/mL),temperature of 30℃,and fermentation period of 10 d.Under these conditions,the conidial yield was 1.672×10^(10)conidia/g,which was about 2~3 times higher than that of the pre-optimization factor test.【Conclusion】The fermentation conditions optimized by the response surface method could obviously increase the sporulation of biocontrol strain AH-1 in solid-state fermentation,which laid a foundation for its large-scale production and preparation of microbial agents.

关 键 词：生防真菌 固体发酵 响应面法 产孢量 

分 类 号：TQ920.6[轻工技术与工程—发酵工程] S476[农业科学—农业昆虫与害虫防治]