液相色谱法在泡菜生物胺含量检测中的应用研究  

Application of High-Performance Liquid Chromatography in the Detection of the Content of Biogenic Amines in Kimchi

作　　者：郝梦甄 HAO Mengzhen(Laixi Inspection and Testing Center,Laixi 266600,China)

机构地区：[1]莱西市检验检测中心,山东莱西266600

出　　处：《现代食品》2024年第20期182-184,共3页Modern Food

摘　　要：本研究通过优化样品前处理和色谱条件,建立了生物胺的标准曲线,结果表明,组胺、酪胺、腐胺、尸胺、色胺、β-苯乙胺、亚精胺和精胺等生物胺在0.10~2000 ng·mL-1的浓度范围内均表现出良好的线性关系,相关系数均在0.995以上。实验结果显示,平均加标回收率范围为76%~100%,相对标准偏差在1.5%~6.1%之间,表明该方法具有较高的准确性和精密度。综上所述,本研究所开发的HPLC法适用于泡菜中生物胺的快速、准确检测,为泡菜的安全性评估和品质控制提供了可靠的技术支持。In this study,the standard curve of biogenic amines was established by optimizing sample pretreatment and chromatographic conditions.The results showed that histamine,tyramine,humutine,psittamine,tryptamine,β-phenylethylamine,spermidine and spermidine showed a good linear relationship in the concentration range of 0.10~2000 ng·mL-1,and the correlation coefficients were all above 0.995.The experimental results show that the average recovery of standard addition ranges from 76%~100%,and the relative standard deviation ranges from 1.5%~6.1%,indicating that the method has high accuracy and precision.In summary,the HPLC method developed in this study is suitable for rapid and accurate detection of bioamines in kimchi,and provides reliable technical support for safety assessment and quality control of kimchi.

关 键 词：高效液相色谱法 泡菜 生物胺 

分 类 号：O657.7[理学—分析化学]