HPLC-MS/MS测定动物源食品中地克珠利、妥曲珠利及其代谢物的残留量  

Determination of Diclazuril,Toltrazuri and Its Metabolites in Foods of Animal Origin by HPLC-MS/MS

作　　者：苏肯明 罗诗泳 朱泳珊 张淯锶 SU Kenming;LUO Shiyong;ZHU Yongshan;ZHANG Yusi(Waltek Services Testing Group Co.,Ltd.,Foshan 528000,China)

机构地区：[1]佛山市沃特测试技术服务有限公司,广东佛山528000

出　　处：《现代食品》2024年第21期163-166,共4页Modern Food

摘　　要：目的:建立一种适用于测定动物源食品中地克珠利、妥曲珠利及其代谢物残留量的方法。方法:样品用乙腈提取后,经PSA净化,液相色谱-串联质谱检测。结果:在1.0~100.0μg·L^(-1)线性范围内,4种待测物相关系数(r^(2))为0.997363~0.999085,水产品、畜禽肌肉、禽蛋类样品检出限均为2.5μg·kg^(-1),内脏样品检出限均为5.0μg·kg^(-1)。当地克珠利、妥曲珠利及其代谢物添加浓度为12.5~250.0μg·kg^(-1)时,在鱼肉样品中回收率为70.01%~88.83%,在鸡肉样品中回收率为70.60%~89.68%,在鸡蛋样品中回收率为74.83%~88.72%;当添加浓度为25.0~500.0μg·kg^(-1)时,猪肝样品回收率为70.84%~85.34%,相对标准偏差(n=6)为0.80%~5.35%。结论:本方法操作简单、灵敏度高,适用于测定动物源食品中地克珠利、妥曲珠利及其代谢物的残留量。Objective:To establish a method for the determination of diclazuril,toltrazuril and their metabolites in animal-derived foods.Method:The samples were extracted with acetonitrile,cleaned up by PSA and detected by liquid chromatography-tandem mass spectrometry.Result:The correlation coefficients(r^(2))of the four analytes were 0.997363~0.999085 in the linear range of 1.0~100.0μg·L^(-1).The detection limits of aquatic products,livestock and poultry muscle,and poultry eggs were all 2.5μg·kg^(-1),and the detection limits of viscera samples were all 5.0μg·kg^(-1).When the concentrations of diclazuril,toltrazuril and their metabolites were added at 12.5~250.0μg·kg^(-1),the recoveries in fish samples were 70.01%~88.83%,in chicken samples were 70.60%~89.68%,and in egg samples were 74.83%~88.72%.When the addition concentration was 25.0~500.0μg·kg^(-1),the recovery rate of pig liver samples was 70.84%~85.34%,and the relative standard deviation(n=6)was 0.80%~5.35%.Conclusion:This method is simple to operate and has high sensitivity.It is suitable for the determination of diclazuril,toltrazuril and their metabolites in animal-derived foods.

关 键 词：地克珠利 妥曲珠利 动物源食品 高效液相色谱-串联质谱 

分 类 号：TS207.3[轻工技术与工程—食品科学]