黑木耳多糖-麦角甾醇复合物制备及体外降胆固醇作用  

Preparation of Auricularia auricula Polysaccharide-Ergosterol Complex and Its Cholesterol-Lowering Effect In vitro

作　　者：李梁 李佳辉 吴佳蔓 王哲铭 贾丰荧 靳雯 云少君[1] 冯翠萍[1] LI Liang;LI Jiahui;WU Jiaman;WANG Zheming;JIA Fengying;JIN Wen;YUN Shaojun;FENG Cuiping(College of Food Science and Engineering,Shanxi Agricultural University,Jinzhong 030801,Shanxi,China)

机构地区：[1]山西农业大学食品科学与工程学院,山西晋中030801

出　　处：《食用菌学报》2024年第6期92-98,共7页Acta Edulis Fungi

基　　金：食用菌山西省科技创新团队(201805D131009);山西农谷建设科研专项项目(SXNGJSKYZX201903);山西省现代农业产业技术体系建设项目(2023CYJSTX09)。

摘　　要：采用反溶剂法制备黑木耳(Auricularia auricula)多糖-麦角甾醇复合物(APE),对其微观形态、结构特征和光、热稳定性进行分析,并研究其对胆固醇在胶束中溶解度的影响。结果表明:麦角甾醇与黑木耳多糖质量比为1∶16时,麦角甾醇的包封率最高;在此条件下制备的APE表面粗糙,呈纤维状,平均粒径为1265 nm。与游离麦角甾醇相比,APE光、热稳定性均较好。APE能够显著降低胆固醇胶束的溶解度。研究结果可为黑木耳多糖和麦角甾醇的进一步开发利用提供参考。Polysaccharides in Auricularia auricula were obtained by water extraction and alcohol precipitation,and then used to prepare A.auricula polysaccharide-ergosterol(APE)complex by the antisolvent method.The complex was observed for microstructure,analyzed for structural characteristics and photothermal stability,and determined for its effect on cholesterol solubility in micelles.The results showed that ergosterol encapsulation efficiency reached the highest when the mass ratio of ergosterol to A.auricula polysaccharides was 1∶16.The APE prepared at this mass ratio had a rough and fibrous surface,and its average particle size was 1265 nm.Compared with free ergosterol,APE showed better photothermal stability.APE significantly reduced the solubility of cholesterol in micelles.These results provided a theoretical basis for further development and utilization of A.auricula polysaccharides and ergosterol.

关 键 词：黑木耳 多糖 麦角甾醇 表征 稳定性 降胆固醇 

分 类 号：TS219[轻工技术与工程—粮食、油脂及植物蛋白工程]