Effects of rhamnogalacturonan-I type polysaccharide purified from Curcuma longa on immunostimulatory and intracellular signaling pathway mechanisms of macrophages  

作　　者：Seung-U Son Eun Hye Choi Kwang-Soon Shin 

机构地区：[1]Department of Food Science and Biotechnology,Kyonggi University,Suwon,16227,Republic of Korea [2]Transdisciplinary Major in Learning Health System,Department of Integrated Biomedical and Life Science,Korea University,Seoul,02841,Republic of Korea

出　　处：《Food Bioscience》2023年第3期1013-1024,共12页食品生物科学（英文）

基　　金：supported by the Basic Science Research Program through the National Research Foundation of Korea(NRF),funded by the Ministry of Education[grant number NRF-2020R1F1A1070996].

摘　　要：In previous study,potent anti-metastatic activities of turmeric-derived pectic polysaccharide(TP)via immune enhancing effects of intravenous and oral administration were evaluated.The present study was designed to elucidate intracellular signaling pathway for macrophage activation by the rhamnogalacturonan-I type polysaccharide(TPE-I)purified from TP.TPE-I increased the production of interleukin(IL)-6,tumor necrosis factor(TNF)-α,and nitric oxide(NO)by RAW264.7 cells.In addition,immunoblotting analysis indicated that TPE-I dose-dependently phosphorylated the mitogen-activated protein kinase(MAPK)and nuclear factor-κB(NF-κB)pathways.Subsequently,TPE-I induced the translocation of activated NF-κB into the nuclei,which was observed by confocal laser scanning microscopy.In the specific inhibitor experiments,cytokines and chemokine secretions were found to be suppressed by BAY11-7082,JNK inhibitor II,and SB20358.Furthermore,the experiments with neutralizing antibodies showed that pattern recognition receptors,such as dectin-1,toll-like receptor(TLR)2,TLR4,cluster of differentiation(CD)14,complement receptors(CR)3,mannose receptor(MR),and scavenger receptor(SR)were involved in cytokine and chemokine secretions by TPE-I in RAW264.7 cells.Consequently,these results suggest that TPE-I phosphorylated the MAPK and NF-κB signaling pathways via mainly the dectin-1,TLR2,TLR4,CD14,CR3,MR,and SR receptors to macrophage,and subsequently induced the secretion of IL-6,TNF-αand NO.

关 键 词：Curcuma longa RHAMNOGALACTURONAN-I MACROPHAGE MAPK and NF-κB 

分 类 号：TS201[轻工技术与工程—食品科学]