Identification and evaluation of antioxidant activity of bioactive casein-derived peptides during in vitro digestion and transepithelial transport in Caco-2 cells  

作　　者：Victor D’Opazo Jorge Calpe Laura Escrivá Tiago Melo Nazareth Giuseppe Meca Carlos Luz 

机构地区：[1]Laboratory of Food Chemistry and Toxicology,Faculty of Pharmacy,University of Valencia,46100,Burjassot,Spain

出　　处：《Food Bioscience》2023年第3期2427-2439,共13页食品生物科学（英文）

基　　金：supported by the Ministry of Economy and Competitiveness(AGL 2016-77610R);by the project Prometeo/2018/126 supported by Generalitat Valenciana;by the pre PhD program of University of Valencia“Atracción de Talento(4690/4690)”.

摘　　要：Dairy products are important sources of bioactive(antibacterial,antithrombotic,antihypertensive,immuno-modulatory,and antioxidative)peptides,that need to be released from the target proteins.In the present study,bovine milk casein protein was hydrolyzed by pancreatin and trypsin(4 and 24 h)to evaluate the hydrolyses efficiency(ninhydrin reaction);to elucidate the sequence of casein-derived peptides(SDS-PAGE,LC-ESI-TOF-MS);and to assess the peptides antioxidant activity(DPPH and ORAC),bioactivity in silico,bioaccessibility after in vitro digestion,and bioavailablility by transepithelial transport in Caco-2 cells.The hydrolysis degree increased with the incubation time,with evident degradation of casein-derived proteins into low molecular weight peptides(<5 kDa)after 4 and 24 h incubation.Pancreatin showed higher hydrolysis degree(91%)than trypsin(21%).An increase in antioxidant activity was observed with increasing incubation time,with higher antioxidant effect observed for trypsin derived peptides(47%reduction of free radicals;32112μM Trolox equivalents)than pancreatin derived peptides(15%reduction;2862μM Trolox equivalents).A total of 69 ca-seins derived peptides were identified after trypsin(33 peptides)and pancreatin(36 peptides)hydrolysis for 24 h;with 26 peptides released fromβ-casein,21 fromα-s1-casein,15 fromα-s2-casein,and 7 from k-casein hy-drolysis.Some of these peptides were described as antioxidant,anti-inflammatory,and antihypertensive.After in vitro digestion 123 peptides were found in gastric(56 peptides),duodenal(43 peptides)and colonic(24 peptides)fraction;while five peptides were bioavailable by crossing the intestinal barrier,with increased peptides’abundance after trypsin hydrolysis treatment.

关 键 词：CASEIN Bioactive peptides Antioxidant activity In vitro digestion BIOACCESSIBILITY BIOAVAILABILITY 

分 类 号：TS201.2[轻工技术与工程—食品科学]