2种贝类鳃暴露于潮间带原油污染的氧化应激效应  

Oxidative Stress Effect in Gills of Two Shellfish Species Exposed to Oil Pollution in Intertidal Zone

作　　者：白石 唐忠伟 杨金生 王益鸣 徐青霞 杨婷婷 潘玉英 庞皓东 谢颖 代金容 毕新建 夏超宇 钱俊 BAI Shi;TANG Zhongwei;YANG Jinsheng(School of Fisheries of Zhejiang Ocean University,Zhoushan 316022;School of Petrochemical Engineering and Environment of Zhejiang Ocean University,Zhoushan 316022,China)

机构地区：[1]浙江海洋大学水产学院,浙江舟山316022 [2]浙江海洋大学石油化工与环境学院,浙江舟山316022 [3]浙江省海洋生态环境监测中心,浙江舟山316021 [4]浙江省海洋开发研究院,浙江舟山316021

出　　处：《浙江海洋大学学报（自然科学版）》2024年第5期413-418,425,共7页Journal of Zhejiang Ocean University：Natural Science

基　　金：浙江省教育厅一般项目(Y202354053);舟山市科技计划项目(2024C61004);浙江省大学生科技创新活动计划(新苗人才计划)项目(2023R411007);浙江省自然科学基金面上项目(LY19D060003);浙江省属高校基本科研业务费项目(2019J00023)。

摘　　要：海洋中发生的原油泄漏事故,会危害海洋生态环境。为了寻找潮间带原油污染生物监测指示生物,研究了2种贝类缢蛏和泥蚶鳃暴露于原油污染的氧化应激效应。在暴露24 h和72 h时,对贝类鳃中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPx)和谷胱甘肽-S转移酶(GST)的活性进行了测定。结果显示:经原油暴露后,缢蛏鳃SOD活性在24 h时受到诱导,在72 h时表现为低浓度(1500 mg·kg^(-1)以下)抑制,高浓度(1500 mg·kg^(-1))诱导;CAT、GPx和GST活性在24、72 h时均受到诱导。泥蚶鳃SOD活性表现出与缢蛏相反的结果;CAT活性在24 h时受到诱导,GST活性表现与其相反,GPx活性则在72 h时受到诱导。研究表明,在原油暴露下,缢蛏的抗氧化能力更强,而原油暴露则总体抑制了泥蚶抗氧化酶的活性。因此,两者均可作为原油污染监测的指示生物,但缢蛏优于泥蚶。缢蛏更适合作为潮间带原油污染监测的指示生物。In the context of marine oil spill incidents,the ecological integrity of the marine environment is jeopardized.In the pursuit of intertidal zone organisms serving as indicators for monitoring crude oil pollution,we investigated the oxidative stress responses in the gills of two bivalve species,Sinonovacula constricta and Tegillarca granosa,exposed to crude oil contamination.The enzymatic activities of superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GPx),and glutathione-S-transferase(GST)in the bivalve gills were assessed after 24 h and 72 h of exposure.The findings indicate that in the S.constricta,SOD activity was induced at 24 h and exhibited concentration-dependent suppression at 72 h,while CAT,GPx,and GST activities were induced at both time points.Conversely,the T.granosa displayed opposing trends in SOD and CAT activities at 24 h,with GST activity showing an inverse pattern,and GPx activity being induced at 72 h.This study underscores the suitability of the S.constricta as a more adept indicator organism for monitoring intertidal zone crude oil pollution.

关 键 词：缢蛏 泥蚶 潮间带 原油污染 抗氧化酶 生物监测 

分 类 号：X171.5[环境科学与工程—环境科学]