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作 者:陈梦元 王文秀 朱远韧 曹越 杨庆 方敏 宫智勇 CHEN Mengyuan;WANG Wenxiu;ZHU Yuanren;CAO Yue;YANG Qing;FANG Min;GONG Zhiyong(School of Food Science and Engineering,Wuhan Polytechnic University,Wuhan 430023,China)
机构地区:[1]武汉轻工大学食品科学与工程学院,武汉430023
出 处:《武汉轻工大学学报》2024年第6期9-18,共10页Journal of Wuhan Polytechnic University
摘 要:桃胶是一种富含多糖的胶质半透明物质,含有多种生物活性物质,具有较高的食用和药用价值。本研究以桃胶为原料,采用高压脉冲电场法提取桃胶多糖,在单因素试验的基础上,利用正交实验确定最佳提取工艺为:脉冲电压60 V,循环次数5次,料液比为1∶100,在此条件下提取的桃胶多糖含量为94.56 g/100g。并通过分子量分析、单糖分析、紫外光谱、傅里叶红外光谱、X-衍射、扫描电镜、刚果红实验、圆二色光谱以及热稳定性测定对桃胶多糖进行表征,结果表明桃胶多糖是由阿拉伯糖、半乳糖、木糖、甘露糖和葡萄糖醛酸组成的具有吡喃糖环的酸性多糖,具有三级螺旋结构的非结晶态聚合物。Peach gum is a gelatinous translucent substance rich in polysaccharides,which has a variety of biological activities,so it has high edible and medicinal value.In this study,peach gum polysaccharides were extracted from peach gum by high voltage pulsed electric field method.On the basis of single factor experiment,the optimal extraction process was determined by orthogonal test as pulse voltage of 60 V,cycle number of 5 times,and solid-liquid ratio of 1:100.Under these conditions,the content of the peach gum polysaccharide extracted was 94.56 g/100 g.Peach gum polysaccharides were characterized by molecular weight analysis,monosaccharide analysis,UV,FT-IR,XRD,SEM,Congo red experiment,CD and TGA.The results show that the gum polysaccharides are acidic polysaccharide with a pyranose ring composed of Ara,Gal,Xyl,Man and Glc-UA,and are amorphous polymer with tertiary helical structure.
分 类 号:TS201.2[轻工技术与工程—食品科学]
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