Single-cell RNA-seq Reveals the Inhibitory Effect of Methamphetamine on Liver Immunity with the Involvement of Dopamine Receptor D1  

作　　者：Jin-Ting Zhou Yungang Xu Xiao-Huan Liu Cheng Cheng Jing-Na Fan Xiaoming Li Jun Yu Shengbin Li 

机构地区：[1]Key Laboratory of National Health Commission for Forensic Sciences,Xi’an Jiaotong University,Xi'an 710061,China [2]National Biosafety Evidence Foundation,Bio-evidence Sciences Academy,Xi'an Jiaotong University,Xi'an 710115,China [3]Department of Cell Biology and Genetics,School of Basic Medical Sciences,Xi’an Jiaotong University Health Science Center,Xi’an 710061,China [4]Department of Respiratory and Critical Care Medicine,The Second Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710004,China [5]National&Local Joint Engineering Research Center of Biodiagnosis and Biotherapy,Precision Medical Institute,The Second Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710004,China [6]OneHealth Technology Company,Xi'an 710000,China

出　　处：《Genomics, Proteomics & Bioinformatics》2024年第4期59-75,共17页基因组蛋白质组与生物信息学报（英文版）

基　　金：supported by grants from the National Natural Science Foundation of China(Grant No.81770798);the Natural Science Foundation of Shaanxi Province(Grant No.2020JQ-083).

摘　　要：Methamphetamine(METH)is a highly addictive psychostimulant that causes physical and psychological damage and immune system disorder,especially in the liver which contains a significant number of immune cells.Dopamine,a key neurotransmitter in METH addiction and immune regulation,plays a crucial role in this process.Here,we developed a chronic METH administration model and conducted single-cell RNA sequencing(scRNA-seq)to investigate the effect of METH on liver immune cells and the involvement of dopamine receptor D1(DRD1).Our findings reveal that chronic exposure to METH induces immune cell identity shifts from IFITM3þmacrophage(Mac)and CCL5þMac to CD14þMac,as well as from FYNþCD4þT effector(Teff),CD8þT,and natural killer T(NKT)to FOSþCD4þT and RORαþgroup 2 innate lymphoid cell(ILC2),along with the suppression of multiple functional immune pathways.DRD1 is implicated in regulating certain pathways and identity shifts among the hepatic immune cells.Our results provide valuable insights into the development of targeted therapies to mitigate METHinduced immune impairment.

关 键 词：Methamphetamine chronic exposure LIVER IMMUNITY Dopamine receptor D1 Single-cell RNA sequencing. 

分 类 号：R575[医药卫生—消化系统]